Experience

Vaccine Production Program

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist

  • Oct 2019 - Present

  Develops methods for analytical characterization of antibodies, and protein and mRNA-based vaccines. • Supervises Associate Scientists o Trains in assays for sample support and development. o Oversees development projects, writing of reports and standard operating procedures. o Approves reports. • Project Coordinator- Acts as liaison between Analytical Development and other departments. o Coordinates project sample support within and between departments. o Presents updates on analytical data to project team and leadership team and participantsin CMC meetings. o Coordinates Manufacturing/Biophysical Risk Assessment (MBRA) for projects. • Writes development reports, standard operating procedures, and journal articles. • Presents development projects at department and division wide meetings. • Performs tech transfer of release assays. • Develops reverse-phase HPLC methods for characterization and identity of mAb and vaccine candidates. o Developed assay separating 4 to 6 hemagglutinin protiens for the determination of the identity and ratio of hemagglutinin in a nanoparticle. o Developing assay to determine sulfation state of mAb. o Developed identity assay to distinguish vaccines that differ by 1 to 2 amino acids. o Developing assay for the determination of the capping efficiency of mRNA. • Performs assays for sample testing o A280, GXII (chip electrophoresis), SEC, HIC



NOVAVAX INC

• Scientist I

  • Nov 2017 - Sep 2019

  Development of analytical methods for the characterization and quantitation of nano-particle based recombinant vaccines. • Works in cross-functional teams. • Performs tech transfer and assay qualification. • Writes SOPs, development reports, qualification protocols and reports. • Lead three projects to develop assays for characterization of recombinant vaccines. • Purity (SDS-PAGE, HPLC- Agilent) • Trypsin sensitivity • Quantitation of residual protein using microBCA. • Performed troubleshooting of assays. • Supervises one Scientist I



MESO SCALE DIAGNOSTICS, LLC.

• Biotechnology Research
• 500 - 600 Employee

• Scientist I

  • Feb 2016 - Oct 2017

  Development of methods for the conjugation, purification and characterization of antibodies, small proteins, oligonucleotides and polysaccharides. • Works in cross-functional teams to develop custom reagents for client assays. • Compose reports for internal projects and for client presentations, write SOPs and Batch Records. • Develop techniques for the analytical characterization of conjugation products and polysaccharides utilizing HPLC (Waters, Agilent) with MALS, RI and UV/vis detection. • Purification of up to 5 mg of conjugated macromolecules. • Supervises and trains 1 research associate in the performance of routine and custom conjugation and analytical techniques



National Cancer Institute

• Construction
• 1 - 100 Employee

• Postdoctoral Fellow

  • Nov 2012 - Feb 2016

  Determination of the mechanism of regulation by the post-translational modification N-acetylglucosamine (O-GlcNac) to understand the role in diabetes and as a cancer biomarker. • Characterized the role of O-GlcNacylated Carboxyl Terminal Domain (CTD) of RNA polymerase II as a nutrient sensor. • Studied the role of O-GlcNacylated NFκB in cell adaptation to hypoxia. • Investigated the function of O-GlcNacylation in promoter proximal pausing. • Managed three projects and formed collaboration that ensured project completion on time. • Designed and executed assay to confirm the CTD as the target for O-GlcNacylation. • Optimized a novel ChIP-IP assay for the detection of GlcNacylated RNA polymerase II on gene promoters.



University of Maryland

• United States
• Higher Education
• 700 & Above Employee

• Graduate Research Assistant

  • Sep 2004 - May 2010

  Kinetic and structural characterization of glutamine-dependent NAD+ synthetase as a potential target for drug development against Mycobacterium tuberculosis. • Developed and optimized molecular cloning, protein expression and purification protocols. o Performed purifications resulting in between 25 to 100 mg of protein. o Developed expertise in the use of FPLC (AKTA) and various chromatography methods. o Performed mutagenesis of NAD+ synthetase. • Developed assays to detect formation of each product of NAD+ synthetase necessitating proficiency in the use of UV/Vis and fluorescence spectroscopy, and HPLC. • Supervised and trained undergraduate and graduate students in the performance of experiments. • Worked with collaborators by providing resources and training in laboratory techniques. • Wrote Standard Operating Procedures (SOP) and reports. • Managed inventory and ordering of laboratory supplies.



Marist College

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Researcher

  • 2003 - 2004

  Developed enzymatic method for biomass processing of paper. • Purified copper containing enzyme laccase. • Steady state characterization and inhibition studies of the laccase redox mediator system. Developed enzymatic method for biomass processing of paper. • Purified copper containing enzyme laccase. • Steady state characterization and inhibition studies of the laccase redox mediator system.



University of Virginia

• United States
• Higher Education
• 700 & Above Employee

• Research Experience for Undergraduates Fellow

  • May 2003 - Aug 2003

  Investigation of enantioselective detection methods for chiral molecules. • Synthesis of fluorescent macrocycles for the enantioselective binding of carboxylates. • Characterization of macrocycles by fluorescence, NMR and mass spectroscopy. Investigation of enantioselective detection methods for chiral molecules. • Synthesis of fluorescent macrocycles for the enantioselective binding of carboxylates. • Characterization of macrocycles by fluorescence, NMR and mass spectroscopy.