Experience

Rijnlands Lyceum Foundation

• Primary and Secondary Education
• 1 - 100 Employee

• Techical Assistant

  • May 2022 - Present



BaseClear

• Netherlands
• Biotechnology Research
• 1 - 100 Employee

• Technician NGS

  • Sep 2021 - Dec 2021



DNAmarkerpoint. IBL. Universiteit Leiden.

• Leiden, Provincie Zuid-Holland, Netherlands

• Lab manager/ Technician

  • Jun 2016 - Dec 2020

  SNP line (LGC Group), ddPCR (Bio-Rad) SNP line (LGC Group), ddPCR (Bio-Rad)



Batavia Biosciences

• Netherlands
• Biotechnology
• 100 - 200 Employee

• Technician Bacterial Vaccines

  • Jul 2013 - May 2016

  Molecular bacteriology lab manager (set up). Pre-clinical studies. Bacterial cell culture. SDS and Native PAGE. DNA and RNA extraction. Qubit assay. BioAnalyzer. PCR. Q-PCR. Sanger Sequencing. ELISA. OPKA. Molecular bacteriology lab manager (set up). Pre-clinical studies. Bacterial cell culture. SDS and Native PAGE. DNA and RNA extraction. Qubit assay. BioAnalyzer. PCR. Q-PCR. Sanger Sequencing. ELISA. OPKA.



Service XS

• Leiden, The Netherlands

• R&D Technician

  • Jan 2012 - Jan 2013

  Next Generation Sequencing (Ion Torrent) Next Generation Sequencing (Ion Torrent)



Delft University of Technology

• Netherlands
• Research Services
• 700 & Above Employee

• Technician (Molecular Biologist)

  • 2005 - Nov 2010

  Protein Expression, SDS PAGE. HPLC. Directed Evolution. Genetic engineering of microorganisms (bacteria and yeast transformation). Plasmid isolation. DNA Amplification, purification and gel extraction. DNA fingerprinting, restriction analyses. DNA sequencing. Protein Expression, SDS PAGE. HPLC. Directed Evolution. Genetic engineering of microorganisms (bacteria and yeast transformation). Plasmid isolation. DNA Amplification, purification and gel extraction. DNA fingerprinting, restriction analyses. DNA sequencing.



DSM

• Netherlands
• Chemical Manufacturing
• 700 & Above Employee

• Technician Genetica

  • Apr 2004 - Nov 2004

  Genetic engineering of microorganisms, bacteria and yeast transformation. Plasmid isolation, Qiaquick Miniprep and Sigma-Aldrich Midiprep. DNA Purification, Qiaquick PCR purification and gel extraction, Zymoclean gel extraction. DNA fingerprinting, restriction analyses. Bioassays. Genetic engineering of microorganisms, bacteria and yeast transformation. Plasmid isolation, Qiaquick Miniprep and Sigma-Aldrich Midiprep. DNA Purification, Qiaquick PCR purification and gel extraction, Zymoclean gel extraction. DNA fingerprinting, restriction analyses. Bioassays.



Universiteit Leiden. Faculteit der Wiskunde en Natuurwetenschappen

• Leiden, The Netherlands

• Analyst

  • Aug 2002 - Sep 2002

  Plant DNA extraction ( CTAB, Qiaquick and Nucleon protocols). Microsatellites. AFLP. Plant DNA extraction ( CTAB, Qiaquick and Nucleon protocols). Microsatellites. AFLP.



Smithsonian Institution. National Museum of Natural History.

• Washington D.C., USA

• Lab assistant

  • Mar 2001 - Apr 2002

  Research on population genetics and systematics of endangered and threatened mammals. Molecular Markers in Population Analyses. DNA extractions from feces, hair and tissues, agarose and polyacrilamide gels, PCR amplifications, Qiaquick purification of PCR samples for DNA sequencing, Cycle sequence reactions, Sephadex of PCR samples for sequence and microsatellite gels, Microsatellite analyses, Operate 377 ABI PRISM automated sequencer, Operate Genescan software for analysis of microsatellite data, Sequencher and Genotyper. Show less



Instituto de Investigaciones Biológicas Clemente Estable

• Montevideo, Uruguay

• Technician

  • 2000 - 2000

  DNA extraction from tissues and feathers, PCR amplifications, Qiaquick purification of PCR samples for DNA sequencing. DNA extraction from tissues and feathers, PCR amplifications, Qiaquick purification of PCR samples for DNA sequencing.



Medical Center

• Montevideo, Uruguay

• Medical lab technician

  • 1992 - 2000

  Blood drain, hematology analyses Blood drain, hematology analyses