PhD candidate "targeting immunosenescence in atherosclerosis"

• Nov 2022 - Present

MSc. internship: strategies aimed at improving microvascular health for ex vivo heart regeneration

• Jan 2022 - Nov 2022

MSc. internship: exploring novel EWSR1-BRCA1 interactions

• Jan 2021 - Aug 2021

The project focused on the verification of a novel EWSR1-BRCA1 interaction and its characterization in terms of transcriptional regulation and DNA repair through homologous recombination. Performed the cloning of plasmids, transformations, mini/maxipreps, PCRs, siRNA and plasmid transfections, co-immunoprecipitations, western blots, PCRs, cell culturing, immunofluorescent staining, fluorescent microscopy, and the generation of a CRISPR-Cas mediated EWSR1-/- cell line a.o. The project focused on the verification of a novel EWSR1-BRCA1 interaction and its characterization in terms of transcriptional regulation and DNA repair through homologous recombination. Performed the cloning of plasmids, transformations, mini/maxipreps, PCRs, siRNA and plasmid transfections, co-immunoprecipitations, western blots, PCRs, cell culturing, immunofluorescent staining, fluorescent microscopy, and the generation of a CRISPR-Cas mediated EWSR1-/- cell line a.o.

Laboratory Technician

• May 2020 - Sep 2020

Customer Service Representative

• Aug 2018 - Jul 2019

Msc. internship the accuracy of virtual bone models for forensic anthropology

• Jan 2017 - Aug 2017

For the development of forensic anthropological identification methods, a contemporary and representative reference database of the Dutch population is required, one which is currently lacking. The set-up of a virtual database derived from patient CT scans could provide a solution, however, any methods derived from a virtual database should be valid on virtual bone models, as well as on physical dry bones, as the latter is most frequently used in forensic anthropology. For this reason, the accuracy of 3D virtual bone models of the os coxae, derived from patient setting CT-scans, when compared to its physical dry bone counterpart was tested. Techniques used were the CT imaging of fleshed cadavers, the maceration for the acquisition of dry bones, segmentation of CT images to create virtual models, and statistical analysis in R performed on inter-landmark distances between modalities Show less

Tutor biomedical sciences and forensic applications

• Feb 2016 - Jan 2017

The project “Forensisch DNA onderzoek: puzzelen met pieken” provided participating senior “HAVO” and “VWO” high school classes with an insight in innovative applications of biology and chemistry within the forensic examination of traces. The project “Forensisch DNA onderzoek: puzzelen met pieken” provided participating senior “HAVO” and “VWO” high school classes with an insight in innovative applications of biology and chemistry within the forensic examination of traces.

Student research assistant

• Jul 2016 - Sep 2016

As a continuation of the bsc. internship project, immunolabeling and confocal imaging was performed of alpha- and beta microtubules and inositol phosphate 3 receptors (IP3) in atrial and ventricular myocytes. Additionally, to verify the expression of, and correct binding of antibodies to, IP3, western blots were performed. As a continuation of the bsc. internship project, immunolabeling and confocal imaging was performed of alpha- and beta microtubules and inositol phosphate 3 receptors (IP3) in atrial and ventricular myocytes. Additionally, to verify the expression of, and correct binding of antibodies to, IP3, western blots were performed.

Bsc. Internship physiological and structural dysfunction in atrial fibrillation

• Apr 2015 - Sep 2015

The aim of the study was to investigate possible alterations in the subcellular structural organization of X-ROS signalling in cardiomyocytes using an experimental animal model of tachy-paced induced atrial fibrillation. The most common techniques performed included the Isolation and fixation of atrial myocytes; membrane staining and confocal imaging of atrial cells with fluorescent dyes; and Immunolabeling and quantification of microtubule structures in intact cells. The aim of the study was to investigate possible alterations in the subcellular structural organization of X-ROS signalling in cardiomyocytes using an experimental animal model of tachy-paced induced atrial fibrillation. The most common techniques performed included the Isolation and fixation of atrial myocytes; membrane staining and confocal imaging of atrial cells with fluorescent dyes; and Immunolabeling and quantification of microtubule structures in intact cells.

Biology and Chemistry tutor

• Nov 2012 - Jun 2015