Experience

Regeneron

• United States
• Biotechnology
• 700 & Above Employee

• R&D Specialist

  • Nov 2021 - Present

  Valhalla, New York, United States

• Research Associate

  • Sep 2018 - Oct 2021

  Valhalla, NY Working in the Protein Biochemistry group, responsible for performing a variety biochemical and biophysical assays to characterize protein biologics to support late-stage development and commercialization efforts.



Memorial Sloan Kettering Cancer Center

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Structural Biology Research Technician

  • Nov 2016 - Sep 2018

  New York, NY ● Designed and optimized protocols for expressing and purifying seven different proteins, allowing for rapid and efficient production of samples for analysis via cryo-electron microscopy. ● Resolved a structure of a transmembrane protein to 9.5 angstrom resolution. ● Generated automated chromatography sequences and methods to minimize the time it takes to load samples. ● Established reputation as the team’s troubleshooter. ● Responsible for lab maintenance (maintain stocks of lab… Show more ● Designed and optimized protocols for expressing and purifying seven different proteins, allowing for rapid and efficient production of samples for analysis via cryo-electron microscopy. ● Resolved a structure of a transmembrane protein to 9.5 angstrom resolution. ● Generated automated chromatography sequences and methods to minimize the time it takes to load samples. ● Established reputation as the team’s troubleshooter. ● Responsible for lab maintenance (maintain stocks of lab materials, troubleshooting broken equipment, cell-line maintenance, preparation of protein stocks, autoclave cycles, etc.) ● Coordinated​ ​with​ ​various​ ​lab​ ​members​ ​to​ ​ensure​ ​timely​ ​project​ ​advancement. ● Summary​ ​of​ ​techniques:​ Size-exclusion chromatography (SEC) (AKTA), fluorescence size-exclusion chromatography (FSEC), affinity chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), cell culture (bacteria, insect, mammalian), absorbance (A280), viral infection (baculovirus), molecular cloning, polymerase chain reaction (PCR), quantitative polymerase chain reaction (qPCR), DNA transfection/transformation, miniprep/maxiprep, dialysis. Show less



Cornell University

• United States
• Higher Education
• 700 & Above Employee

• Graduate Researcher

  • Sep 2015 - May 2016

  Ithaca, NY ● Built​ ​a​ ​numerical​ ​model​ ​to​ ​simulate​ ​the​ ​transport​ ​and​ ​capture​ ​of​ ​charged​ ​colloidal​ ​particles​ ​in​ ​depth​ ​filtration​ ​via​ ​MATLAB. ● Re-engineered​ ​an​ ​existing​ ​model​ ​for​ ​air​ ​filtration​ ​to​ ​account​ ​for​ ​factors​ ​governing​ ​aqueous​ ​particle​ ​motion​ ​(e.g.​ ​low​ ​Reynolds​ ​number fluid​ ​dynamics,​ ​Coulomb​ ​interactions,​ ​lubrication​ ​forces,​ ​Brownian​ ​motion,​ ​membrane​ ​fouling)​ ​to​ ​calculate​ ​particle​ ​trajectories. ●… Show more ● Built​ ​a​ ​numerical​ ​model​ ​to​ ​simulate​ ​the​ ​transport​ ​and​ ​capture​ ​of​ ​charged​ ​colloidal​ ​particles​ ​in​ ​depth​ ​filtration​ ​via​ ​MATLAB. ● Re-engineered​ ​an​ ​existing​ ​model​ ​for​ ​air​ ​filtration​ ​to​ ​account​ ​for​ ​factors​ ​governing​ ​aqueous​ ​particle​ ​motion​ ​(e.g.​ ​low​ ​Reynolds​ ​number fluid​ ​dynamics,​ ​Coulomb​ ​interactions,​ ​lubrication​ ​forces,​ ​Brownian​ ​motion,​ ​membrane​ ​fouling)​ ​to​ ​calculate​ ​particle​ ​trajectories. ● Conceived and established a method for calculating particle capture probability, paving the way for predicting filter performance. ● Acquired an extensive theoretical understanding of depth filtration processes and the key parameters that govern filter performance. ● Received​ ​the​ ​Outstanding​ ​MEng.​ ​Project​ ​Award​ ​from​ ​Cornell​ ​Chemical​ ​&​ ​Biomolecular​ ​Engineering​ ​Department. Show less



Momentive

• United States
• Chemical Manufacturing
• 700 & Above Employee

• Specialty Fluids Research & Development Intern

  • May 2015 - Aug 2015

  Tarrytown, NY ● Studied​ ​the​ ​rate​ ​of​ ​foam​ ​collapse​ ​as​ ​a​ ​function​ ​of​ ​formulation​ ​composition,​ ​method​ ​of​ ​preparation,​ ​and​ ​the​ ​manner​ ​of​ ​foam​ ​production for​ ​an​ ​antifoaming​ ​product​ ​with​ ​previously​ ​undocumented​ ​properties. ● Proposed​ ​a​ ​novel​ ​mechanism​ ​of​ ​antifoaming​ ​action​ ​by​ ​applying​ ​the​ ​results​ ​of​ ​my​ ​experiments​ ​to​ ​general​ ​antifoaming​ ​theory, which furthered Momentive's understanding of the underlying mechanisms governing… Show more ● Studied​ ​the​ ​rate​ ​of​ ​foam​ ​collapse​ ​as​ ​a​ ​function​ ​of​ ​formulation​ ​composition,​ ​method​ ​of​ ​preparation,​ ​and​ ​the​ ​manner​ ​of​ ​foam​ ​production for​ ​an​ ​antifoaming​ ​product​ ​with​ ​previously​ ​undocumented​ ​properties. ● Proposed​ ​a​ ​novel​ ​mechanism​ ​of​ ​antifoaming​ ​action​ ​by​ ​applying​ ​the​ ​results​ ​of​ ​my​ ​experiments​ ​to​ ​general​ ​antifoaming​ ​theory, which furthered Momentive's understanding of the underlying mechanisms governing the behavior of this particular product. ● Publication: Koczo, K., Howie, D., Policello, G. A., Truong, L., Brown, W. L., and Naue, J. A., “Foam Control of Trisiloxane Alkoxylate Superspreaders with Modified Trisiloxane Alkoxylates: A Mechanistic Study,” Pesticide Formulation and Delivery Systems: 37th Volume, Formulations with Ingredients on the EPA’s List of Minimal Concern, ASTM STP1602, R. K. Edlund, Ed., ASTM International, West Conshohocken, PA, 2018, pp. 63–77, http://dx.doi.org/10.1520/ STP1602201601352. ● Recognized on the following poster presentation by Dr. Kalman Koczo: "Mechanism of antifoams for trisiloxane alkoxylate solutions" presented at the 2016 ISAA '16 conference in San Francisco. Show less



The Johns Hopkins University

• United States
• Higher Education
• 700 & Above Employee

• Research Lab Assistant

  • Sep 2013 - May 2015

  Baltimore, MD ● Developed/tested​ ​DNA​ ​strand​ ​displacement​ ​reaction​ ​systems​ ​via​ ​numerical​ ​simulation​ ​and​ ​subsequent​ ​experimental​ ​validation. ● Designed​ ​a​ ​numerical​ ​method​ ​in​ ​MATLAB​ ​to​ ​convert​ ​raw​ ​fluorescence​ ​data​ ​into​ ​meaningful​ ​results​ ​(concentration​ ​vs.​ ​time),​ ​which significantly​ ​increased​ ​experimental​ ​throughput​ ​and​ ​overall​ ​efficiency,​ ​allowing​ ​for​ ​quicker​ ​results. ● Summary​ ​of​ ​techniques:​ MATLAB, DNA… Show more ● Developed/tested​ ​DNA​ ​strand​ ​displacement​ ​reaction​ ​systems​ ​via​ ​numerical​ ​simulation​ ​and​ ​subsequent​ ​experimental​ ​validation. ● Designed​ ​a​ ​numerical​ ​method​ ​in​ ​MATLAB​ ​to​ ​convert​ ​raw​ ​fluorescence​ ​data​ ​into​ ​meaningful​ ​results​ ​(concentration​ ​vs.​ ​time),​ ​which significantly​ ​increased​ ​experimental​ ​throughput​ ​and​ ​overall​ ​efficiency,​ ​allowing​ ​for​ ​quicker​ ​results. ● Summary​ ​of​ ​techniques:​ MATLAB, DNA strand-displacement reactions, ​quantitative polymerase chain reaction (qPCR),​ ​fluorescence​ ​spectroscopy,​ polyacrylamide gel-electrophoresis (​PAGE). ● Publication: Fern, J., Scalise, D., Cangialosi, A., Howie, D., Potters, L., Schulman, R. "DNA Strand-Displacement Timer Circuits,” ACS Synth. Biol., 2017, 6 (2), pp 190-193, http://dx.doi.org/10.1021/acssynbio.6b00170. ● Recognized on the following poster presentations by Dr. Rebecca Schulman and PhD candidate Dominic Scalise: - “Continuously Active Molecular Circuits” presented at the 2015 Molecular Programming Project Conference in San Francisco. - “Sustained Enzyme Free Molecular Circuits” presented at the 2015 Biophysical Society Conference in Baltimore. - “Synthetic DNA Delay Circuits for Temporal Coordination” presented at the 2015 SEED Conference in Boston. Show less



Regeneron

• United States
• Biotechnology
• 700 & Above Employee

• Therapeutic Protein Formulations Intern

  • May 2013 - Aug 2013

  Tarrytown, NY ● Analyzed​ ​the​ ​effects​ ​of​ ​various​ ​excipients/buffers,​ ​and​ ​combinations​ ​thereof,​ ​on​ ​aqueous​ ​monoclonal​ ​antibody​ ​aggregation. ● Proposed​ ​a​ ​change​ ​to​ ​the​ ​current​ ​product​ ​formulation​ ​and​ ​backed​ ​up​ ​my​ ​claims​ ​with​ ​concrete​ ​experimental​ ​results. ● Summary​ ​of​ ​techniques:​ High-performance liquid chromatography (​HPLC),​ ​rheometer,​ ​buffer​ ​preparation,​ ​absorbance (A280).