Experience

Dynacyte Biosciences

• Singapore
• Biotechnology Research
• 1 - 100 Employee

• Applied Biologist

  • Mar 2022 - Present

  (1) Developed a data augmentation pipeline for increasing dataset size and automated labeling(2) Trained and tested a multi-scale, multi-object detection network (YOLO) for identifying cells and bacteria in microscopic images via transfer learning approach; achieved detection accuracy of 99%(3) Prototyped real-time object detection/recognition using a webcam(4) Create a python-based simulator for monitoring, control and simulating processes in bioreactors (1) Developed a data augmentation pipeline for increasing dataset size and automated labeling(2) Trained and tested a multi-scale, multi-object detection network (YOLO) for identifying cells and bacteria in microscopic images via transfer learning approach; achieved detection accuracy of 99%(3) Prototyped real-time object detection/recognition using a webcam(4) Create a python-based simulator for monitoring, control and simulating processes in bioreactors



Nanyang Technological University Singapore

• Singapore
• Higher Education
• 700 & Above Employee

• Postdoctoral Researcher

  • Oct 2021 - Feb 2022

  Design and Fabrication of scaffolds as implants for spinal cord injury(1) Fabricated and evaluated gelatin-cyclodextrin scaffolds for (a) 3D cell culture and (b) controlled protein delivery (2) Developed photocurable 3D scaffolds for implantation into injured regions of spinal cords in rat models. (3) RCULA certified to handle laboratory rats and mice Design and Fabrication of scaffolds as implants for spinal cord injury(1) Fabricated and evaluated gelatin-cyclodextrin scaffolds for (a) 3D cell culture and (b) controlled protein delivery (2) Developed photocurable 3D scaffolds for implantation into injured regions of spinal cords in rat models. (3) RCULA certified to handle laboratory rats and mice



Singapore University of Technology and Design (SUTD)

• Singapore
• Higher Education
• 700 & Above Employee

• Doctoral Researcher

  • Sep 2016 - Aug 2021

  Thesis: Modelling Cancer Cell Migration in Tumor-on-Chip Platforms and Future Integration with Machine Intelligence(1) Developed a Gelatin Methacrylate (GelMA) based tumor mimicking model and formulated a method to tune matrix stiffness alone, to independently modulate cancer cell motility.(2) Correlated cancer cell migration patterns by performing cell tracking on high-resolution, time-lapse images.(3) Performed computation and statistical analysis of tracking data using custom MATLAB code.(4) Trained and evaluated a deep generative network for accurately positioning nuclei from cytoskeletal organization

• Graduate Teaching Assistant

  • May 2017 - Dec 2017

  Managed laboratory for the course "30.203 Topics in Biomedical and Healthcare Engineering"(1) Conducted lab sessions and evaluated student performance for a class of 30(2) Taught and guided students to work with fundamental biology techniques such as cell culture, designing drug delivery systems and paper-based colorimetric sensors.



PICKCELL LAB

• Product Development Intern

  • May 2017 - Aug 2017

  Fabrication of membrane based microwell system for culturing mammalian cells in 3D(1) Evaluated the efficacy of Polyvinyl alcohol (PVA) and Polyethylene Teraphthalate (PET) substrates for fabricating membrane microwells (2) Utilized the process of capillary force lithography (CFL) to replicate structures from silicone based molds to light curable resins coated onto PVA or PET substrates(3) Successfully optimized standards for fabricating microwells of well defined features with minimal mechanical damage.



Johnson & Johnson

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Project Management Intern

  • Sep 2015 - Dec 2015

  (1) Market research and analysis of quality of care of patients undergoing knee replacement surgeries in Singapore's public and private hospitals. (2) Developed and presented solutions to reduce the length of stay of patients undergoing knee surgeries in Singapore based hospitals.(3) Worked as medical affairs assistant on a collaborated project with Singapore Rugby Union (SRU) where I was involved with the medical team in the preparation of documents and supporting evidence in order to gain accreditation for a Standard First Aid course.



National University of Singapore

• Singapore
• Higher Education
• 700 & Above Employee

• Graduate Student Researcher- Part time

  • Jun 2014 - Dec 2014

  (1) Fabricated and optimized formulation of Poly-lactide-co-glycolide (PLGA) and Polyethylene glycol (PEG) based water-in-oil polymeric emulsion films for controlled protein delivery.(2) Investigated its ability to deliver model proteins in vitro in a controlled manner compared to formulations with other emulsifiers like Span-80. (1) Fabricated and optimized formulation of Poly-lactide-co-glycolide (PLGA) and Polyethylene glycol (PEG) based water-in-oil polymeric emulsion films for controlled protein delivery.(2) Investigated its ability to deliver model proteins in vitro in a controlled manner compared to formulations with other emulsifiers like Span-80.



SRM University (Sri Ramaswamy Memorial University)

• India
• Education Management
• 400 - 500 Employee

• Summer Research Intern

  • May 2012 - Jun 2012

  (1) Synthesised Diopside (MgCaSi2O6) using industrial waste product, rice straw(2) Fabricated Chitosan/Diopside scaffolds for bone tissue engineering (3) Characterised scaffolds using Dynamic Light Scattering (DLS), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive Analysis (EDX) and Fourier Transformed Infrared Analysis (FTIR). (1) Synthesised Diopside (MgCaSi2O6) using industrial waste product, rice straw(2) Fabricated Chitosan/Diopside scaffolds for bone tissue engineering (3) Characterised scaffolds using Dynamic Light Scattering (DLS), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive Analysis (EDX) and Fourier Transformed Infrared Analysis (FTIR).



Bioklone Biotech Private Limited

• India
• Biotechnology Research
• 1 - 100 Employee

• Summer Research Intern

  • Jun 2011 - Jun 2011

  (1) Mammalian Cell culture- Maintenance of Hybridoma cell lines(2) Antibody purification by Affinity Chromatography followed by characterization using Sodium Dodecyl Sulphate- Polyacrylamide Gel Electrophoresis (SDS-PAGE), Western Blot, Dot Blot and Enzyme-linked Immunosorbent Assay (ELISA) techniques. (1) Mammalian Cell culture- Maintenance of Hybridoma cell lines(2) Antibody purification by Affinity Chromatography followed by characterization using Sodium Dodecyl Sulphate- Polyacrylamide Gel Electrophoresis (SDS-PAGE), Western Blot, Dot Blot and Enzyme-linked Immunosorbent Assay (ELISA) techniques.



CSIR - Central Leather Research Institute

• India
• Research Services
• 100 - 200 Employee

• Research Intern

  • Jan 2011 - Jan 2011

  Fabricated keratin scaffolds for application in tissue engineering Fabricated keratin scaffolds for application in tissue engineering