Experience

Light Chain Bioscience | Novimmune SA

• Switzerland
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Assistant

  • Nov 2016 - Present

  -Support early drug discovery in Phage Display Screening Unit -Contribute to the generation and characterization of antibody libraries that will be used for discovery of monoclonal and/or bispecific drug candidate -Simultaneously work on two different activities: expansion of KL-body primary libraries for the bispecific antibody platform and construction of libraries for the lead optimization of candidates from several antibody discovery programs. Construction of libraries: -Perform DNA preparation (minipreps, maxipreps),Perform DNA amplifications by PCR, Large scale molecular cloning for library construction, Large scale transformation of bacteria by electroporation Production, purification and titration of bacteriophage, DNA and protein sequence analysis Antibody in vitro discovery Perform phage selections and screening, Set-up assays (Cell Insight, ELISA),Perform Cell culture, Hit characterization: Cell assays, ELISA, FACS Optimization of new techniques and protocols. Analyze and present results. Maintain good relationship with lab suppliers and external contractors. Show less



University of Geneva

• Switzerland
• Research Services
• 700 & Above Employee

• Research Assistant

  • Sep 2015 - Sep 2016

  -Contribute to the characterization of the Hydra regeneration, budding, aging mechanisms. In charge of manual in Situ Hybridization and Electroporation of animals. Cloning, sequencing, riboprobes synthesis, RNA extraction, cDNA synthesis, RT-PCR, qPCR. -Hydra culture: Helping on the daily washing of the “Hydra” cultures and preparation of Hydra medium on request. -Participate to Lab activities including lab stock & routine equipment maintenance. -Write reports. -Contribute to the characterization of the Hydra regeneration, budding, aging mechanisms. In charge of manual in Situ Hybridization and Electroporation of animals. Cloning, sequencing, riboprobes synthesis, RNA extraction, cDNA synthesis, RT-PCR, qPCR. -Hydra culture: Helping on the daily washing of the “Hydra” cultures and preparation of Hydra medium on request. -Participate to Lab activities including lab stock & routine equipment maintenance. -Write reports.



Light Chain Bioscience | Novimmune SA

• Switzerland
• Biotechnology Research
• 1 - 100 Employee

• Research Assistant in Molecular Biology

  • Jan 2014 - Aug 2015

  -New mission in Phage Display Screening Unit . -New mission in Phage Display Screening Unit .



Light Chain Bioscience | Novimmune SA

• Switzerland
• Biotechnology Research
• 1 - 100 Employee

• Research Assistant in Molecular Biology (Temporary – 5 months mission)

  • Feb 2013 - Jun 2013

  -Support early drug discovery in Phage Display Screening Unit -Contribute to the generation and characterization of antibody libraries that will be used for discovery of monoclonal and/or bispecific drug candidate -Perform plasmid preparation and DNA amplification by PCR -Large scale molecular cloning for library construction -Transformation of bacteria and purification of bacteriophage -DNA sequence analysis -Support early drug discovery in Phage Display Screening Unit -Contribute to the generation and characterization of antibody libraries that will be used for discovery of monoclonal and/or bispecific drug candidate -Perform plasmid preparation and DNA amplification by PCR -Large scale molecular cloning for library construction -Transformation of bacteria and purification of bacteriophage -DNA sequence analysis



Merck Healthcare

• Germany
• Pharmaceutical Manufacturing
• 700 & Above Employee

• • Oct 2008 - Jan 2013

  -Support research and clinical biomarkers projects under QMS guidelines.-Clinical studies by analyzing human blood and/or tumors samples.-RNA & miRNA extraction (Qiacube) and QC (Nanodrop, Bioanalyser 2100,SpectraMax, QiaXel) from various tissues(Whole Blood, Cells, tumors, plasma, FFPE). -Design, realize gene expression analysis using different technologies: Microarrays (Affymetrix, Nugen), qPCR (TaqMan, SyBr Green, Exiqon) automation (Qiacube, Biomek FXP, GeneTitan).Analysis of qPCR results ( SDS,StatMiner,Excel).-Laboratory analysis report writing ( SOP, LAP, FOP ).-Technical development and establishment of news technologies: microRNA extraction and detection (real-time PCR,array) in the laboratory.-Training, helping and supervising co-workers to improve processes (technicians, students, temporary employes).- Interacting with scientists from multidisciplinary fields. Show less

• • Jan 2006 - Sep 2008

  -Discovery and development of therapeutic antibodies. -Support minipreps (BioRobot 8000) and sequencing service (3730xL). -Cell culture , transfections, proteins expression and purification.-Training in cell culture techniques in order to set up in vitro assays to evaluate mAb activity.-Microarray experiments using the Illumina platform, with RNA from endothelial cells (BEND5), microglia (EOC20), neuroblastoma (N2a, SH-SY5Y) and oligodendrocytes to look for biomarkers which might provide a cell-based assay to follow the activity of the Epo variants.-Molecular biology support for the anti-Fractalkine and anti-GM-CSF projects (Cloning mAb cDNAs from selected hybridomas).-Molecular biology support for the Epo splice variants project (design of Taqman probes and invesitigation by qPCR of the tissue distribution of human Epo splice variants).-Performance of qPCR and microarray experiments according to needs and priorities of other groups. Show less



Serono SA

• Geneva Area, Switzerland

• Senior Technician II in Molecular Biology Department

  • Jan 1998 - Dec 2005

  -Responsible for quantitative RT-PCR platform (ABI 7700 and ABI 7900HT fast). -Designing Primers with PrimerExpress and search of gene sequences on Internet. -Clonage, PCR, mutagenesis( Gateway, Blunt end), genotyping. -Maintenance of plasmids and primers databases. -Laboratory equipment maintenance. -Process improvement by reducing process time, reducing cost and increasing High Throughput -Responsible for quantitative RT-PCR platform (ABI 7700 and ABI 7900HT fast). -Designing Primers with PrimerExpress and search of gene sequences on Internet. -Clonage, PCR, mutagenesis( Gateway, Blunt end), genotyping. -Maintenance of plasmids and primers databases. -Laboratory equipment maintenance. -Process improvement by reducing process time, reducing cost and increasing High Throughput



GSK

• United Kingdom
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Senior Technician I in Biochemistry + Genomics Departments

  • Mar 1991 - Dec 1997

  Senior Technician I (6 years): -In charge of sequencing platform with 8 sequencers (ABI 377, 377XL, 3700). Technician II in Biochemistry Department (1 year): -Culturing yeast cells under sterile conditions. In vivo radioactive labelling of yeast lipids. ELISA, Electrophoresis, Western Blot, SDS-Page, HPLC Senior Technician I (6 years): -In charge of sequencing platform with 8 sequencers (ABI 377, 377XL, 3700). Technician II in Biochemistry Department (1 year): -Culturing yeast cells under sterile conditions. In vivo radioactive labelling of yeast lipids. ELISA, Electrophoresis, Western Blot, SDS-Page, HPLC



University and Cantonal Hospital Geneva

• Geneva Area, Switzerland

• Technician in HUG and CMU

  • Sep 1990 - Feb 1991

  -6 months laboratory assistant (Preparation of radiotracers) Nuclear medicine department HUG. -6 months laboratory assistant (Mouse Cancer Models, cell culture) CMU. -6 months laboratory assistant (Preparation of radiotracers) Nuclear medicine department HUG. -6 months laboratory assistant (Mouse Cancer Models, cell culture) CMU.



Battelle

• United States
• Research Services
• 700 & Above Employee

• Technician in Neuropharmacology Department

  • Nov 1983 - Nov 1990

  -Receptor Binding, Recapture, Liberation and Metabolism of neuromediator. PK/PD modelling. -In vivo pharmacology validation (Mice, Rats, Gerbils) under GLP conditions. -Cerebral ischemia Gerbils models. -Psychopharmacology. -Receptor Binding, Recapture, Liberation and Metabolism of neuromediator. PK/PD modelling. -In vivo pharmacology validation (Mice, Rats, Gerbils) under GLP conditions. -Cerebral ischemia Gerbils models. -Psychopharmacology.



Hospital Chambery (France)

• Chambéry Area, France

• Technician in Clinical Laboratory of Biochemistry and Microbiology

  • Nov 1982 - Sep 1983

  -Clinical Laboratory of Biochemistry and Microbiology -Clinical Laboratory of Biochemistry and Microbiology