Experience

Verily

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Senior Scientist (Life Sciences)

  • Aug 2019 - Present



Draper

• United States
• Defense and Space Manufacturing
• 700 & Above Employee

• Senior Member of the Technical Staff, Bio Systems and Tissue Engineering

  • Sep 2016 - Aug 2019

  Task Lead, Organ on a chip system. Developed a high-throughput liver-on-a-chip platform recapitulating in vivo flow conditions for long term cell culture. Design and empirical calculations for material choices for optimal oxygen delivery and sustain long-term function of primary hepatic cultures. Developed primary multi-cellular liver models, for applications in NASH, Fatty acid disease modeling focused on cellular interactions, immune responses, and fatty acid accumulation. Demonstrated concepts of drug metabolism in low volume, microfluidic cultures Lead a team of scientists, interfacing with multiple clients, managing budget, monthly and quarterly reports, and milestone deliveries. Show less



Massachusetts General Hospital

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Assistant in Bioengineering

  • May 2016 - Sep 2016

  Independent researcher developing skin and immune system-on-chip platforms to aid in allergenic compound screening in vitro. Protein-based nucleic acid delivery systems for rapid manufacture and packaging nucleic acids.

• Research Fellow

  • Oct 2010 - Apr 2016

  Research Scholar, Center for Engineering at Medicine: Develop research strategies, grant writing, mentoring junior members and technicians, and independently execute projects.1. 3D biomimetic liver sinusoid constructs for predicting physiology and toxicity:a) Isolation and co-culture of non-parenchymal cells (sinusoidal endothelial, stellate, and Kupffer cells) and hepatocytes from rat and human liversb) Primary hepatocyte - liver sinusoidal endothelial cell co-culture for 4 weeks, demonstrating high viability, stability, secretions, and enzymatic functionsc) Multi-cellular in vitro rat primary co-culture system endothelial, stellate, and Kupffer cells with hepatocytes to mimic the liver function and inflammatory responses. d) A novel low-volume two-chamber microfabricated platform to mimic in vivo media-cell ratio for drug metabolism, metabolite formation, and resulting toxicity in a liver-cancer cell model.e) Microfluidic liver sinusoid model incorporating primary hepatocytes and non-parenchymal cells to mimic physiology for drug metabolism applicationsf) A liver-kidney inter-organ interaction model to mimic organ interactions by drug metabolism and metabolites2. Developed an ultra-sensitive, rolling circle amplification-based assay for Islet Cell Autoantibody measurement and early detection of Type 1 Diabetes3. Collaborations: 1) Differential effects of HCV and HIV in hepatic fibrogenesis, 2) Function of long non-coding RNAs in hepatic fibrosis, and 3) NASH pathogenesis Show less



Harvard Medical School

• United States
• Higher Education
• 700 & Above Employee

• Research Fellow

  • Oct 2010 - Sep 2016

  Research Fellow at the Center for Engineering in Medicine with co-appointments at Harvard Medical School, Massachusetts General Hospital, and Shriners Hospital for Children, Boston, MA Research Fellow at the Center for Engineering in Medicine with co-appointments at Harvard Medical School, Massachusetts General Hospital, and Shriners Hospital for Children, Boston, MA



Shriners Children's

• Hospitals and Health Care
• 700 & Above Employee

• Research Staff

  • Oct 2010 - Sep 2016

  Research Fellow at the Center for Engineering in Medicine with co-appointments at Harvard Medical School, Massachusetts General Hospital, and Shriners Hospital for Children, Boston, MA Research Fellow at the Center for Engineering in Medicine with co-appointments at Harvard Medical School, Massachusetts General Hospital, and Shriners Hospital for Children, Boston, MA



Rensselaer Polytechnic Institute

• United States
• Higher Education
• 700 & Above Employee

• Visiting Scholar

  • Jun 2010 - Oct 2010

  My research involved the investigation of toxicity and effect on metabolic pathways of mammalian cells upon exposure to various nanoscale materials. Specifically, we studied the effect of zinc oxide and silica nanoparticles of varying sizes on a variety of cell lines, including progenitor stem cells. Our findings reveal that nanoscale materials though toxic at higher concentrations can mediate the differentiation of stem cells at significantly lower concentrations.

• Graduate Student

  • Aug 2005 - May 2010

  Synthesis and assembly of nanoscale biological-material hybrids and their use for selective cellular delivery 1. Silica nanoparticle-protein conjugates for cytoplasmic delivery and function in cytoplasma) Delivering proteins to specifically recognize cellular components for deactivation or activation of key cellular components and signaling pathways influencing cell behavior and directing cell fateb) Developed a GFP complementation system for monitoring and directing nanomaterial mediated protein delivery to cellular organelles (mitochondria)2. Protein-directed synthesis of nanotube-nanoparticle conjugates. Prepared carbon nanotube – protein – silver nanoparticle conjugates with multiple functions3. Structure, function, and stability of enzymes attached to nanomaterials: carbon nanotubes, nanoparticlesa) Tubulin-mediated assembly of carbon nanotubes to form supramolecular structuresb) Manipulation of carbon nanotubes conjugated with tubulin microtubules for transport on kinesin tracks 4. Paint based functional surfaces containing carbon nanotube-enzyme conjugates for active decontamination Show less



Osmania University, Hyderabad, India

• Hyderabad Area, India

• Undergraduate Student

  • Aug 2001 - Jun 2005

  My project in Pogaku’s lab involved the development of an overall equation for determining the thermal conductivity of non-newtonian fluids. Attempts have been made to study the limitations of existing typical correlations and develop a general equation for thermal conductivity to account for various ranges of fluids. The developed equation was then fitted to available correlations and validated with corresponding experiments. My project in Pogaku’s lab involved the development of an overall equation for determining the thermal conductivity of non-newtonian fluids. Attempts have been made to study the limitations of existing typical correlations and develop a general equation for thermal conductivity to account for various ranges of fluids. The developed equation was then fitted to available correlations and validated with corresponding experiments.



Everest Organics Limited

• Hyderabad Area, India

• Intern

  • May 2003 - Jun 2003

  During my internship, I was trained in process monitoring and product optimization using chromatography techniques including TLC, HPLC, and GC. Later, I worked in the production plant, overseeing the preparation of a variety of pharmaceutical compounds and quality control tests. A detailed report has been submitted as a part of the internship and is currently in use at the factory for guiding staff and new interns. During my internship, I was trained in process monitoring and product optimization using chromatography techniques including TLC, HPLC, and GC. Later, I worked in the production plant, overseeing the preparation of a variety of pharmaceutical compounds and quality control tests. A detailed report has been submitted as a part of the internship and is currently in use at the factory for guiding staff and new interns.