Order Picker

• Jun 2017 - Present

Research Intern

• Nov 2015 - Jun 2016

Master Thesis InternshipProject: “Glycosylation of anti-citrullinated protein antibodies (ACPA) in rheumatoid arthritis”Techniques: ELISA, FACS, Protein purification (FPLC-ÄKTA), Mammalian cell culture, Western Blotting, SDS-PAGEDescription: ACPA and IgG were isolated from ACPA+ RA patient plasma or synovial fluid with affinity chromatography (FPLC) and the success of the isolations was confirmed by ACPA IgG ELISA. Next, we generated ACPA and IgG immune complexes and tested their binding to FcγR-transfected CHO cell lines. The expression of the Fcγ receptors on the cells surface was previously confirmed with flow cytometry. After the detachment of the bound immune complexes, their Fc glycosylation was analysed by mass spectrometry. Next to this, we also studied by Western Blotting whether ACPA-IgG subclasses have a higher molecular weight compared to non-ACPA IgG, due to Fab glycosylation.

Research Intern

• Feb 2015 - Jun 2015

Project: ’’Identification of human polyoma viruses in salivary gland carcinoma’’Since HPyVs and high risk HPVs are closely related and the latter has proven to have an association with head and neck cancers, we wanted to investigate whether HPyVs can be detected in salivary gland carcinomas. If a significant subset of positive cases in our cohort was found, it could be concluded that HPyVs are associated with this cancer type. The viruses that we tried to identify were HPyV6, HPyV7 and MCPyV and the method used was PCR with primers specific for each of the aforementioned viruses’ sequences.

Bachelor Internship

• Jan 2013 - Oct 2013

Bachelor Thesis Project“RAPD Application for Molecular Detection of Lactobacillus Pentosus B281”It has been shown that the bacterial strain Lactobacillus pentosus B281 can be considered a potential probiotic microorganism. Its genome has not been fully characterized. Therefore, the development of a molecular technique to identify B281, is necessary. In the current thesis a RAPD PCR methodology was developed, a technique which does not require prior knowledge of the target sequence. 50 random primers were used, 10 base pairs in length, which amplified parts of genomic DNA of L. pentosus B281 as well as parts of other L. pentosus strains. The PCR products were analyzed using agarose gel electrophoresis. PCR products that resulted in at least 4 bands were chosen for further analysis. The unique bands of the desired products were extracted and used as a DNA insert in a bacterial cloning vector, which then was amplified and sequenced. After the sequence analysis of the DNA inserts, the BLAST program was used to examine the homology to other sequences stored in databases.

QC Analyst

• Jun 2012 - Aug 2012

3-month internship in the drug Quality Control department at pharmaceutical company Pharmathen International Chemical Quality Control▪ HPLC▪ Dissolution method▪ Drug substance identification with UV / IRMicrobiological Quality Control▪ Check for potential microbial growth on drugs (yeast, E. Coli)▪ Check for potential microbial growth on raw materials (yeast, E. Coli, Salmonella,Staphylococcus Aureus, Pseudomonada)http://www.pharmathen.com/home/home-27.htm?lang=en