Graduate Research Assistant

• Apr 2020 - Present

Team IEB - Research in Intestinal Epithelial Barrier in Autoimmune Diseases - Processing the whole human gut for cryopreservation/biobanking - Animal experiments: EAE and SLE mouse models - Stainings and Imaging: histology and Immunofluorescence stainings - Molecular Biology Techniques: intestinal permeability assays, sample preparation for RNAseq analysis, genomic DNA isolation and genotyping, protein biochemistry, relative gene expression analysis - Cell Culture: monocyte to macrophage differentiation - Lab Management: ordering, delivery handling, keeping inventories, writing SOPs, lab and equipment maintenance Show less

Graduate Research Intern

• Oct 2019 - Mar 2020

The Schiebel Lab - Yeast: yeast two-hybrid screening and cell cycle synchronization - Molecular Biology Techniques: cloning, site-directed mutagenesis, cassette PCR, SDS-PAGE and western blot analysis, split-GFP assay - Imaging: fluorescence microscopy and image analysis The Schiebel Lab - Yeast: yeast two-hybrid screening and cell cycle synchronization - Molecular Biology Techniques: cloning, site-directed mutagenesis, cassette PCR, SDS-PAGE and western blot analysis, split-GFP assay - Imaging: fluorescence microscopy and image analysis

Research Assistant

• Sep 2017 - May 2019

Cellular Immunology Lab - Animal Experiments: cell isolation from mouse organs and bone marrow - Cell Culture: Bone marrow to MDSC differentiation - Molecular Biology Techniques: Molecular cloning, genomic DNA and RNA isolations, polymerase chain reactions, sequencing analysis, retroviral infection, SDS-PAGE and western blot analysis, flow cytometry analysis and cell sorting Cellular Immunology Lab - Animal Experiments: cell isolation from mouse organs and bone marrow - Cell Culture: Bone marrow to MDSC differentiation - Molecular Biology Techniques: Molecular cloning, genomic DNA and RNA isolations, polymerase chain reactions, sequencing analysis, retroviral infection, SDS-PAGE and western blot analysis, flow cytometry analysis and cell sorting

• 2013 - 2016

Founder Member, 2013Supervisory Board, 2013-2014Event Manager, 2014-2015- Organizing culinary events, workshops, and restaurant visits- Organizing gastronomic trips and seminars- Publishing a gastronomy journal

• Sep 2014 - Jan 2015

Human Genetics Lab at Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center- Sample collection from volunteered Behçet and FMF patients- PBMC isolation from whole blood, protein isolation from PMBC samples, western blot analysis

• Aug 2012 - Aug 2013

Head of Club, 2012-2013Supervisory Board, 2013-2014- Weekly meetings and journal clubs- Organizing international conferences- Organizing student club parties

Student Intern

• Jan 2015 - Feb 2015

Comparative Evolutionary Biology Studies - Learning the basics of Python and R programming languages Comparative Evolutionary Biology Studies - Learning the basics of Python and R programming languages

Student Intern

• Jun 2014 - Jul 2014

Aziz Sancar Institute of Istanbul University Hospital - Immune profiling, flow cytometry analysis and cell sorting Aziz Sancar Institute of Istanbul University Hospital - Immune profiling, flow cytometry analysis and cell sorting