Experience

Captor Therapeutics SA

• Poland
• Biotechnology Research
• 1 - 100 Employee

• R&D Specialist

  • Oct 2022 - Present

  Protein Engineering Group Protein Engineering Group



Pure Biologics

• Poland
• Biotechnology Research
• 1 - 100 Employee

• Biotechnology Specialist

  • Jan 2019 - Sep 2022

  Protein Group Specialist• recombinant protein expression in E. coli system• recombinant and native proteins purification on a milligram to the gram scale (>95% purity)• high-quality protein antigens production (immunization grade)• developing of protein isolation and purification methods• identification, modification and analysis• DSF-based protein stability optimization• protein immobilization• HTS - high-throughput expression screening of recombinant proteins in E. coli• Quality Control of recombinant proteins (SDS-PAGE, SEC-HPLC, DSF, WB and more) Show less

• Junior Biotechnology Specialist

  • Oct 2017 - Dec 2018

  Contractual Research DepartmentProtein Purification Team• recombinant and native proteins purification on a milligram scale to the gram scale (>95% purity)• developing of protein isolation and purification methods



Malopolska Centre of Biotechnology at Jagiellonian University

• Kraków, woj. małopolskie, Polska

• Research Biologist

  • Mar 2016 - Feb 2017

  Malopolska Centre of Biotechnology at Jagiellonian University Department of Structural Biology X-Ray Crystallography Laboratory • optimization of protein overproduction conditions • cloning with restriction enzymes and LIC (RF cloning) • PCR site-directed mutagenesis • Plasmid DNA isolation • protein chromatography – IMAC, AC, IEC, SEC (gravity flow chromatography, GE ÄKTAprime plus, GE ÄKTA Purifier) • protein analysis – SDS-PAGE, Western Blot • protein refolding experiments • improving stability conditions screening for labile proteins • protein crystallization by sitting-drop format of vapor diffusion method: conditions screening and optimization Show less



Malopolska Centre of Biotechnology at Jagiellonian University

• Kraków, Woj. Małopolskie, Polska

• Intern

  • Jul 2015 - Sep 2015

  Malopolska Centre of Biotechnology at Jagiellonian University Department of Structural Biology X-Ray Crystallography Laboratory • protein expression optimization • protein purification – IMAC, AC, IEC, SEC (gravity flow chromatography, GE ÄKTAprime plus, GE ÄKTA Purifier) • protein purification in small scale and pull-down assays using Dynabeads Magnetic Beads • determination of protein purity by SDS-PAGE • determination of protein concentration by Bradford assay or UV method • assisting in protein crystallization preparation (sitting-drop format of vapor diffusion method), optimization of crystallization conditions • plasmid DNA isolation • cloning with restriction enzymes Show less



Wroclaw University of Science and Technology

• Poland
• Higher Education
• 700 & Above Employee

• Bachelor Engineering thesis

  • Mar 2014 - Feb 2015

  Faculty of ChemistryDepartment of Bioorganic Chemistry"Analysis of Enzymatic Activity of Helicobacter pylori Glutamine Synthetase"• preparation of buffers, solutions and reagents for protein purification and enzymatic activity assay• chemically competent cells preparation and transformation by heat shock method• amplification and isolation of plasmid DNA• heterologous expression of recombinant Helicobacter pylori glutamine synthetase in E. coli strain• protein purification (IMAC, SEC)• FPLC GE ÄKTAprime plus system• determination of protein purity by SDS-PAGE• enzymatic activity analysis: determination of Km parameters for GS substrates, optimal pH and temperature Show less

• Trainee (laboratory assistant)

  • Oct 2013 - Feb 2014

  [research laboratory as an elective course]• heterologous expression of untagged urease from Helicobacter pylori in E. coli strains• isolation and purification of urease (salting out, chromatographic methods, dialysis) for further crystallization• determination of protein purity by SDS-PAGE• urease activity assay• FPLC GE ÄKTAprime plus system



Pure Biologics

• Poland
• Biotechnology Research
• 1 - 100 Employee

• Intern

  • Jul 2014 - Sep 2014

  • preparation of buffers and other solutions for proteins purification • preparation of media and LB-agar plates (bacterial system expression) and media for mammalian cell lines • protein expression screening and optimization • SDS-PAGE, Western Blot and Dot Blot • selection of scFv antibodies against various antigens by Phage Display technology • purification of antibodies by affinity chromatoghaphy (protein A) and IMAC • mammalian cell culture techniques: passage of cells growing in suspension and adherent cells • cytotoxicity tests – MTT assay • molecular biology techniques: isolation of plasmids; preparation of chemically competent bacterial cells; DNA digestion with restriction enzymes and analysis by agarose gel electrophoresis Show less



Wojskowy Ośrodek Medycyny Prewencyjnej Wrocław

• Wrocław, woj. dolnośląskie, Polska

• Trainee

  • Sep 2013 - Sep 2013



Wroclaw University of Science and Technology

• Poland
• Higher Education
• 700 & Above Employee

• Trainee

  • Jul 2013 - Aug 2013

  Faculty of Chemistry Department of Bioorganic Chemistry • microbiological cultures • substrate-induced expression of ureases from Sporosarcina pasteurii (Bacillus pasteurii) and Proteus mirabilis • isolation and purification of native proteins (salting out, chromatographic methods, dialysis) • determination of protein purity by SDS-PAGE • urease activity assay • FPLC GE ÄKTAprime plus system Faculty of Chemistry Department of Bioorganic Chemistry • microbiological cultures • substrate-induced expression of ureases from Sporosarcina pasteurii (Bacillus pasteurii) and Proteus mirabilis • isolation and purification of native proteins (salting out, chromatographic methods, dialysis) • determination of protein purity by SDS-PAGE • urease activity assay • FPLC GE ÄKTAprime plus system