Rogelio Alvarado
Graduate Student Researcher at The Graduate School at Northwestern University- Claim this Profile
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Bio
Experience
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The Graduate School at Northwestern University
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United States
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Higher Education
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1 - 100 Employee
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Graduate Student Researcher
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Sep 2022 - Present
- Construction and cloning of Plasmid Libraries with genetic mutations - Protein expression, purification, and characterization of virus-like-particles (VLPs) - Protein structure characterization via ChimeraX - SDS-PAGE Gel Electrophoresis for Protein Expression - Construction and cloning of Plasmid Libraries with genetic mutations - Protein expression, purification, and characterization of virus-like-particles (VLPs) - Protein structure characterization via ChimeraX - SDS-PAGE Gel Electrophoresis for Protein Expression
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Northeastern Illinois University
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United States
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Higher Education
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700 & Above Employee
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STEM Ambassador (Title III)
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Sep 2020 - Aug 2023
- Build a community of STEM students virtually - Provide technical advice and support to STEM students- Speed networking with students, teachers, and faculty- Establish practical STEM demonstrations- Used Canva and Google apps to help with marketing events
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Undergraduate Student Researcher
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Apr 2020 - Aug 2023
- Summer research grant awarded by Student Center for Science Engagement- Performed excel quality control check for deep sequenced RNAseq libraries- Use databases including Wormbase, Ensembl, and BLAST to analysis gene list to find human orthologs and tissue expression patterns- Develop hypotheses and interpretations from differential gene expression profiles for TDP-43 expressing C. elegans- Review procedures to properly care for worms and manipulate their genome
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Stagehand
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Feb 2016 - Aug 2023
- Assisted in setting up audio, lighting, and technical equipment- Lead student employees to complete event set-ups- Managed finances, scheduling, and ticketing for events
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Northwestern University - The Feinberg School of Medicine
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United States
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Higher Education
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700 & Above Employee
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Research Assistant
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Mar 2021 - Dec 2021
- Prepare buffer solutions needed for day to day lab experiments - Conduct Bicinchoninic Acid (BCA) Assay on cancer cell lines to measure protein concentrations - Run Western Blot immunoassay on cancer cell lines to identify protein expression - Conduct qRTPCR, gene expression analysis - Design and Order Primers for experiments - Data analysis utilizing shell script and R program, and Bioconductor packages - Prepare buffer solutions needed for day to day lab experiments - Conduct Bicinchoninic Acid (BCA) Assay on cancer cell lines to measure protein concentrations - Run Western Blot immunoassay on cancer cell lines to identify protein expression - Conduct qRTPCR, gene expression analysis - Design and Order Primers for experiments - Data analysis utilizing shell script and R program, and Bioconductor packages
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Education
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Northwestern University
Doctor of Philosophy - PhD, Interdisciplinary Biological Sciences -
Northeastern Illinois University
Bachelor's degree, Biology, General