Experience

Locanabio, Inc.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Sr Development Associate II

  • Sep 2021 - Present

  Developed Affinity, AEX, and Harvest/TFF unit operations for AAV purification and formulation Designed, executed, and analyzed multiple DOE experiments to maximize full capsid and yield at harvest Scaled platform process to 200L Pilot Lab, including multiple successful campaigns Tech Transfer and CDMO collaboration Supported experimental work with analytics, ddPCR and ELISA Developed Affinity, AEX, and Harvest/TFF unit operations for AAV purification and formulation Designed, executed, and analyzed multiple DOE experiments to maximize full capsid and yield at harvest Scaled platform process to 200L Pilot Lab, including multiple successful campaigns Tech Transfer and CDMO collaboration Supported experimental work with analytics, ddPCR and ELISA



Novartis Gene Therapies

• United States
• Biotechnology Research
• 700 & Above Employee

• Process Science Engineer II

  • Aug 2019 - Aug 2021

  Designed, executed, and performed data analysis on experiments for next generation AAV process development Developed scale down model of manufacturing process to support pipeline candidate screening and research material needs across the Novartis GTx network Processed adherent and suspension HCCF at scales ranging from 25mL Shake Flasks up to 200L Stirred Tank Reactor Collaborated with project managers to establish timelines, coordinate testing plans, manage sample tracking, and deliver purified virus Established data tracking spreadsheet to collect and trend product quality data Operated large scale processing to scale up process development work, generate material for Tox studies, and support investigations Show less



bluebird bio

• United States
• Biotechnology Research
• 300 - 400 Employee

• Associate Scientist II, Vector Process Development

  • Feb 2018 - Jul 2019

  Execute routine production of LVV at bench and manufacturing scale, processing from cell culture to drug product Doubled weekly bench-scale production capacity by streamlining AKTA methods Revamped bench-scale and manufacturing-scale batch records to align with Process Justification and Control Strategy Implemented database to capture and trend in-process data across bench- and manufacturing-scale productions, improved stakeholder accessibility to data Execute routine production of LVV at bench and manufacturing scale, processing from cell culture to drug product Doubled weekly bench-scale production capacity by streamlining AKTA methods Revamped bench-scale and manufacturing-scale batch records to align with Process Justification and Control Strategy Implemented database to capture and trend in-process data across bench- and manufacturing-scale productions, improved stakeholder accessibility to data



Dimension Therapeutics

• United States
• Biotechnology

• Research Coop

  • Jul 2015 - Dec 2015

  Helped develop a scalable, robust process to purify AAV using depth filtration, sterile filtration, and column chromatography Evaluated and optimized chromatography methods for various resins to compare yield and scalability and establish parameters Helped develop a scalable, robust process to purify AAV using depth filtration, sterile filtration, and column chromatography Evaluated and optimized chromatography methods for various resins to compare yield and scalability and establish parameters



Alexion Pharmaceuticals, Inc.

• United States
• Biotechnology Research
• 700 & Above Employee

• Purification Associate

  • Jan 2014 - Jun 2014

  My responsibilities were primarily concerned with bioreactor harvest processing, including depth filtration, hollow fiber ultrafiltration/diafiltration, and column chromatography. I did extensive work developing an NUPAGE method for the quickest, cleanest image development using Western Blot and Sypro Ruby. My responsibilities were primarily concerned with bioreactor harvest processing, including depth filtration, hollow fiber ultrafiltration/diafiltration, and column chromatography. I did extensive work developing an NUPAGE method for the quickest, cleanest image development using Western Blot and Sypro Ruby.