Experience

CINCINNATI VA MEDICAL CENTER RESEARCH
• United States
• 1 - 100 Employee
• Clinical Research Coordinator

  • Jul 2022 - Present

Cengage Group
• United States
• E-Learning Providers
• 700 & Above Employee
• Customer Technical Support

  • Jun 2020 - Jul 2022


University of Chicago Hospitals

• United States
• Medical Practices
• 1 - 100 Employee

• Research Assistant

  • 2008 - Aug 2015

  •Prepare human brain tissue homogenates, and analyze samples via Western blot. •Organize and manage the IBC protocol, ACUP protocol, Carlson Biosafety animal colony, and CIS facilities mouse colony management. •Data Analysis and data preparation for publication and grants. •Oversee and train students in laboratory safety and experimental technique. •Prepare human and mouse tissue homogenates for experiments and Western blot analysis. •Use of scrapie cell culture system to predict the scrapie infectivity of different mutations in prion protein. •Use of fluorescence resonance energy transfer to predict altered molecular association of prion protein in prion disease. •Drug Intraperitoneal injections into mice for ongoing experiments. •Patient DNA extraction from blood and human tissue; followed by DNA sequence analysis to genetically screen for Creutzfeldt-Jakob disease. •Preparation of 300 μm and 700 μm mouse brain sections using a vibratome with Ringer solution, carbogen (95% O2/5%CO2), and ice •Gonadotropin releasing hormone (GnRH) neuron secretion analyses are performed in the presence of veratridine, which opens Na+channels causing depolarization of the neuron. Then analysis is done via radioimmunoassay (RIA) using GnRH antibody radiolabeled with I125 to quantify the amount of hormone being released by the neuron. •Protein purification using baculovirus expression system and pMAL protein fusion and purification system. •Organized and maintained Neuroblastoma, SMB, HEK and COS7 cell culture •Bacterial and yeast transformation, molecular cloning, DNA sequencing analysis, UV Mutagenesis, gene disruption, site directed mutagenesis, Western blots, and enzyme kinetic assays. •DNA purification, Restriction Enzyme digest, Polymerase Chain Reaction, and RT-PCR •Experience with tissue fixation, embedding, histology, operation of the transmission electron microscope (TEM) and Confocal Microscope. •ELISA and antibody purification, NMR, and IR spectroscopy Show less