Mariangelli Orlando
Field Application Scientist - Technical Sales at MaxCyte, Inc.- Claim this Profile
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English Native or bilingual proficiency
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Spanish Native or bilingual proficiency
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French -
Topline Score
Bio
Melissa Crisp
Maria worked heavily in supporting early drug discovery programs through work in cloning, protein expression, and cell line development. She was involved in mammalian expression of novel therapeutic proteins. She is very comfortable in the use of automation and spent a lot of time working closely with the automation group to streamline the small-scale transient transfection process. Together we were able to bridge the gap between cell line development and automation and have a setup where small scale transient transfections could be done in a high throughput format.
Alexus Smith
Maria was one of my supervisor while I was interning for the Summer at Neurocrine Biosciences. Together we worked on providing tissue culture support for our different teams. That included generating membrane preparations for compound binding assays, transfections of stable suspension cell lines, as well as cryogenic storage of cells. Also she taught me molecular biology and cloning, as well as DNA sequencing. She was very personable and so excited to teach me new techniques. Maria was also enthusiastic about interaction with her colleagues at all levels. I hope one day I will be able to work in this industry again with her.
Melissa Crisp
Maria worked heavily in supporting early drug discovery programs through work in cloning, protein expression, and cell line development. She was involved in mammalian expression of novel therapeutic proteins. She is very comfortable in the use of automation and spent a lot of time working closely with the automation group to streamline the small-scale transient transfection process. Together we were able to bridge the gap between cell line development and automation and have a setup where small scale transient transfections could be done in a high throughput format.
Alexus Smith
Maria was one of my supervisor while I was interning for the Summer at Neurocrine Biosciences. Together we worked on providing tissue culture support for our different teams. That included generating membrane preparations for compound binding assays, transfections of stable suspension cell lines, as well as cryogenic storage of cells. Also she taught me molecular biology and cloning, as well as DNA sequencing. She was very personable and so excited to teach me new techniques. Maria was also enthusiastic about interaction with her colleagues at all levels. I hope one day I will be able to work in this industry again with her.
Melissa Crisp
Maria worked heavily in supporting early drug discovery programs through work in cloning, protein expression, and cell line development. She was involved in mammalian expression of novel therapeutic proteins. She is very comfortable in the use of automation and spent a lot of time working closely with the automation group to streamline the small-scale transient transfection process. Together we were able to bridge the gap between cell line development and automation and have a setup where small scale transient transfections could be done in a high throughput format.
Alexus Smith
Maria was one of my supervisor while I was interning for the Summer at Neurocrine Biosciences. Together we worked on providing tissue culture support for our different teams. That included generating membrane preparations for compound binding assays, transfections of stable suspension cell lines, as well as cryogenic storage of cells. Also she taught me molecular biology and cloning, as well as DNA sequencing. She was very personable and so excited to teach me new techniques. Maria was also enthusiastic about interaction with her colleagues at all levels. I hope one day I will be able to work in this industry again with her.
Melissa Crisp
Maria worked heavily in supporting early drug discovery programs through work in cloning, protein expression, and cell line development. She was involved in mammalian expression of novel therapeutic proteins. She is very comfortable in the use of automation and spent a lot of time working closely with the automation group to streamline the small-scale transient transfection process. Together we were able to bridge the gap between cell line development and automation and have a setup where small scale transient transfections could be done in a high throughput format.
Alexus Smith
Maria was one of my supervisor while I was interning for the Summer at Neurocrine Biosciences. Together we worked on providing tissue culture support for our different teams. That included generating membrane preparations for compound binding assays, transfections of stable suspension cell lines, as well as cryogenic storage of cells. Also she taught me molecular biology and cloning, as well as DNA sequencing. She was very personable and so excited to teach me new techniques. Maria was also enthusiastic about interaction with her colleagues at all levels. I hope one day I will be able to work in this industry again with her.
Credentials
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Clinical Laboratory Scientist Trainee
California Department of Public HealthDec, 2011- Nov, 2024 -
Technologist in Molecular Biology ASCP(MB)
American Society for Clinical Pathology (ASCP)Jan, 2007- Nov, 2024
Experience
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MaxCyte, Inc.
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United States
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Biotechnology
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1 - 100 Employee
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Field Application Scientist - Technical Sales
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Sep 2021 - Present
Jan 2023 - Present In addition to being the FAS for the Southwest USA, my duties have expanded to cover the South and Latin America for as a Technical Sales / Application Scientist professional. Sep 2021 - Jan 2023 Application Scientist supporting customers in the Southwest USA, Latin and South America. Providing technical and troubleshooting support to customers of Maxcyte's Scalable Transfection Systems as well as working with customers to identify and implement solutions for process improvements throughout the transfection workflow, including pre- and post-electroporation. Responsible for conducting product demonstrations, end-user training and troubleshooting, both during the evaluation phase and post-sale. Show less
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Neurocrine Biosciences
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Greater San Diego Area
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Senior Scientist
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Oct 2017 - Sep 2021
Integral member of the in vitro pharmacology team supporting multiple new target screening and lead optimization campaigns by providing and maintaining cell lines as well as bringing new technologies in to improve our current systems. I manage the everyday lab management, from prioritizing and completing everyday tasks to becoming a point person for all lab-related issues (troubleshooting, planning, triage and administration). I take charge of lab operations and use best practices to ensure high performance of our lab. I established relationships with key suppliers, scheduled demos and negotiated pricing for the lab equipment that was crucial to increase and optimize output of our lab. I was able to purchase instruments while saving on our overall capital budget, and in turn, doubling the output and efficiency of our lab. My day-to-day lab management includes: Inventory control, laboratory assets management (preventative maintenance and calibration schedules setup and maintenance, routine break/fix), and vendor management. Show less
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Takeda
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Japan
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Pharmaceutical Manufacturing
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700 & Above Employee
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Associate Scientist
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Jan 2015 - Apr 2017
Support molecular biology and cell culture efforts to express a variety of proteins such as mAbs, fusion proteins, and antigens for in vitro and in vivo research studies. Support the generation of reagent cell lines for in vitro cell-based assays. Generated proteins and materials from both transient and stable expression using both HEK293 and CHO. I was involved in the cell line development process for a lead molecule, which involved screening multiple clones in fed-batch shake flasks, performing 60 generation stability studies on the final clones, and summarizing the data in an IND report. Actively involved in implementing new methods with a goal of increasing protein titers, improving throughput of expression, or reducing timelines. This was done by improving our transfection protocols and also by FACS sorting high expressing populations. I also obtained training on FACS Sorting on the BD Aria II. Show less
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Eli Lilly and Company
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United States
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Pharmaceutical Manufacturing
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700 & Above Employee
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Senior Biologist
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2012 - Feb 2015
Support early drug discovery programs through work in cloning, expression, and cell line development. Heavily involved in mammalian expression of novel therapeutic proteins. Use automation and HTS in both cell line development and screening. Work closely with antibody discovery, protein engineering and purification groups at Lilly as projects move from research to the clinic. Support early drug discovery programs through work in cloning, expression, and cell line development. Heavily involved in mammalian expression of novel therapeutic proteins. Use automation and HTS in both cell line development and screening. Work closely with antibody discovery, protein engineering and purification groups at Lilly as projects move from research to the clinic.
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Amgen
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United States
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Biotechnology Research
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700 & Above Employee
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Senior Associate Scientist
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Mar 2001 - 2011
Senior Associate Scientist – Protein Science Developed high throughput assays, generated expression vectors, expression cell lines, and designed antigens in order to support the different therapeutic areas. Evaluated and developed new imaging assays to meet project needs, and also explored other approaches to overcome the inadequacy of current assays. Heavily involved in GPCR and Ion channel expression and assay development and screening. Work closely with antibody discovery, protein chemistry and purification groups at Amgen as projects move from research to the clinic. Lab Operations In addition to my lab duties, I was also responsible for laboratory management, such as lab operations, placing work orders, maintaining lab equipment, keeping up with service contracts. Implemented a Min/Max system to prevent lab from running out of essentials. Trained others on how to properly run lab instruments and maintained user logs to monitor equipment usage and or malfunctions. Established relationships with key vendors. Negotiated pricing for lab equipment and supplies. Worked closely with vendors, scheduling demos; I was able to purchase instruments saving on overall budget, extra money was allocated to additional equipment. Show less
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University of Miami
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United States
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Higher Education
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700 & Above Employee
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Research Associate
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1998 - 2000
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Centers for Disease Control and Prevention
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United States
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Government Administration
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700 & Above Employee
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Research Associate
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1997 - 1998
Part of a five-member research team investigating smallpox. Developed expertise ranging from nucleic acid technologies (PCR, restriction digests, etc.) to tissue culture, protein expression, and immunology (ELISA, HA, HI, Western Blotting). Acquired extensive experience in both acrylamide and agarose gel electrophoresis. Performed all the appropriate training and protocols required to maintain a BL3 level laboratory. Part of a five-member research team investigating smallpox. Developed expertise ranging from nucleic acid technologies (PCR, restriction digests, etc.) to tissue culture, protein expression, and immunology (ELISA, HA, HI, Western Blotting). Acquired extensive experience in both acrylamide and agarose gel electrophoresis. Performed all the appropriate training and protocols required to maintain a BL3 level laboratory.
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Marriott Hotels
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United States
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Hospitality
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700 & Above Employee
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Spanish Instructor
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1996 - 1997
Teach the housekeeping staff basic English skills to enable them to communicate with their employer. Also separate course to teach the hotel managers basic Spanish skills. Teach the housekeeping staff basic English skills to enable them to communicate with their employer. Also separate course to teach the hotel managers basic Spanish skills.
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Education
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California Lutheran University
MBA in Organizational Behavior from California Lutheran University -
University of California San Diego
Clinical Trial Mgt and design -
Georgia State University
BS, Microbiology, Chemistry -
Georgia Institute of Technology
Biology -
University of Puerto Rico, Río Piedras
High School Diploma