Research Technician

• Jul 2018 - Present

Biomedical Lab Assistent

• Dec 2017 - Mar 2018

Biomedical Lab Assistent

• May 2016 - Sep 2017

Diagnostic research with immunological assays for determining Lyme's disease. Helped with the development of a new test to diagnose Lyme's disease faster. Diagnostic research with immunological assays for determining Lyme's disease. Helped with the development of a new test to diagnose Lyme's disease faster.

Intern

• May 2015 - Dec 2015

Research on IL-17a production and the development of Th17 cells. A characteristic hallmark of auto-immune diseases is inflammation which is associated with local increase of cytokines and interleukins. Direct or indirect inhibition of the inflammatory response could be realized by the use of small molecule drugs. Here it would prove beneficial to use a cell model instead of primary cells. Therefore two cell lines were screened. EL-4 which is a mouse T-lymphoblast cell line and CCRF-CEM which is a human T-lymphoblast cell line. Used techniques: DNA/RNA isolation, (q-)PCR, cloning, flow cytometry, cell culture, ELISA.

Intern

• Nov 2013 - Jan 2015

HSP90 dependent folding of CFTR: CFTR is a transmembrane protein that is important for chlorine transport along the epithelium and plays a big role in cystic fibrosis. In literature it is stated that interaction with HSP90, a chaperone protein, is crucial for correct folding of CFTR. However other ABC-transporter proteins do not need to interact with HSP90. We wanted to see if the addition of mutations and deletions could alter the protein structure and make CFTR a more stable protein able to fold independently of HSP90. Pulse chase experiments were used to see how HSP90 is involved in the folding process of CFTR. Additionally I used PyMoL to analyze proteïne structure between several proteins related to CFTR. Used techniques: Western-blot, cloning, PCR, pulse-chase, PyMoL.

Intern

• Nov 2012 - Aug 2013

University Medical Centre Utrecht, Department of Cell Biology/Cell Screening Center, the Netherlands. The Chemical Biology of Lysosomal Cell Death: Developing a digitonin based assay to see if anti-cancer compounds could initiate lysosomal cell death. Lactate dehydrogenase assay would provide insight into whether digitonin permeabilizes only the outer cell membrane. A cathepsin assay would confirm if the lysosomal membrane is permeabilized by the compound. Additionally western blot was used to check if LAMP was still present in control cells treated with the same amount of digitonin as the drug treated cells. Used techniques: Western-Blot, cell culture, in-vitro assays (LDH, Cathepsin Assay).

Intern

• Jan 2012 - Jun 2012

Ubiquitin Ligases and Cancer: Identification of GBF1 as a novel substrate of βTrCP through use of immune precipitation and western blotting. Studied GBF1 phosphorylation, ubiquitylation and degradation by using immune precipitation followed by in vitro ubiquitylation assay. And by using cell cycle inhibiting drugs to elucidate when GBF1 is degraded. Site directed mutagenesis was also used to create a lentiviral construct to see which phosphodegron sites are necessary for GBF1 degradation.Used techniques: Western-Blot, immunoprecipitation, cell culture, cloning, ubiquitylation assay, PCR.