Hrshita Gowda
Graduate Research Assistant at Duke University Pratt School of Engineering- Claim this Profile
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Bio
Experience
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Duke University Pratt School of Engineering
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United States
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Higher Education
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300 - 400 Employee
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Graduate Research Assistant
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Jan 2021 - Present
Human microbiome Machine learning and drug discovery Computational biology Human microbiome Machine learning and drug discovery Computational biology
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Northeastern University
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United States
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Higher Education
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700 & Above Employee
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Graduate Student Council Representative
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Sep 2019 - Dec 2020
Identify common issues across departments and programs and communicate to COS committee. Represent COS graduate students to COS Advocate needs and priorities of students to COS Dean’s Office Facilitate open communication between students and COS Identify common issues across departments and programs and communicate to COS committee. Represent COS graduate students to COS Advocate needs and priorities of students to COS Dean’s Office Facilitate open communication between students and COS
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Nektar Therapeutics
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Biotechnology
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300 - 400 Employee
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Intern- Biologics
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Jun 2019 - Dec 2019
Developed CIEX based flowthrough FPLC platform to achieve separation of protein differing in degree of pegylation by overloading while using conductivity as a variable. Preliminary experience with filtration platforms as part of process characterization studies: UFDF; TFF Evaluation of stability of purification buffer using pH and conductivity as standpoints. Laboratory scale resin scouting to facilitate host cell protein removal in process intermediates. Comprehensive knowledge of protein purification. Screening of commercially available HCP ELISA kits to provide an estimate of host cell protein levels present in IL-2. Host cell protein quantification in process intermediates utilizing ELISA based platform methods. Protein concentration determination by SOLO VPE Data Analytics and its significance to PAT. Show less
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Biocon
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India
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Biotechnology Research
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700 & Above Employee
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Senior Research Associate
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Jul 2016 - Aug 2018
Analytical and characterization SME for anti-diabetic peptide: Worked on structural characterization of proteins and bio-pharmaceuticals in the form of sequencing, impurity characterization, process validation with an objective of establishing bio-similarity for successful filling and launching of anti-diabetic peptide in ROW markets. Provided regulatory support (including sequencing, impurity characterization, process validation and bio-similarity) for successful filling and launching a long acting basal Insulin analogue in regulated markets of Japan,EU and US.Analytical and characterization SME for complex polypeptide: Provided regulatory support(including sequencing, process validation and bio-similarity) for filling ANDA in regulated markets including USA, Canada and successfully launching the product in Uruguay. Design of experiments, executing protocols and activities related to force degradation studies of biopharmaceutical molecules involving biosimilars that assisted in establishing the comparativedegradation across biosimilars and lot-to-lot consistency of the products upon utilization of stateof-art modern chromatographic techniques, high definition Mass spectrometry (ESI-MS)/MALDItechniques but not limited to.Managed the critical role of strategically procuring quality imbibed materials for the smooth functioning of the laboratory.Quality system implementation in the team to develop a quality integrated research platform. Outsource Study Coordinator: Successfully coordinated with different vendors (international as well as domestic) to ensure quality integrated scientific data to be presented to regulators. Show less
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Research Associate
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Sep 2014 - Jul 2016
Mass spectrometer based characterization of Insulin and its analogs: Primary and secondary structure determination using different Mass spectrometer platforms(ESI-MS, LTQ-Orbitrap,MALDI TOF) Chromatography: RP-HPLC, IEX,SECCharacterization of whole cell protein samples including HCP by systematic approachbased on In-GEL platform and analyzing the isolated proteins by MALDI TOF-MS.
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Intern @ Molecular Characterisation Laboratory
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Apr 2014 - Sep 2014
Impurity profiling of Biologics using Chromatography and Mass spectrometry.
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inSTEM, NCBS
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Bengaluru Area, India
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Research trainee
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Feb 2013 - May 2013
Worked on the effects of FMRP and its phosphomutant S499D on psd95 under serum starved conditions. Worked on the effects of FMRP and its phosphomutant S499D on psd95 under serum starved conditions.
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Education
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Duke University Pratt School of Engineering
Doctor of Philosophy - PhD, Bioengineering and Biomedical Engineering -
Northeastern University
Master of Science - MS, Biotechnology -
M.V.J. College Of Engineering
Bachelor's Degree, Biotechnology