Experience

Institute of Molecular Biology (IMB)

• Germany
• Research
• 100 - 200 Employee

• Doctoral Student

  • Sep 2022 - Present

  Mainz, RP, Germany Mainz, RP, Germany



The Investigator Taiwan

• Taiwan
• Biotechnology Research
• 1 - 100 Employee

• Project manager, Reviewer, Writer

  • Nov 2018 - Present

  • More than 20 articles posted on The Investigator Taiwan.• Creating more than 45K views on WordPress.

• Director of Academic Section

  • Aug 2020 - Jul 2021

  • From Structural biology, Microbiology, Metabolism, to Cancer biology, bringing cutting-edge life science knowledge to first-line scientists.



National Yang Ming University

• Taiwan
• Research
• 300 - 400 Employee

• Research Assistant

  • Sep 2020 - Aug 2022

  • First author publication: Multiomics reveal the central role of pentose phosphate pathway in resident thymic macrophages to cope with efferocytosis-associated stress. Cell Reports · Jul 12, 2022Thymic-resident macrophages, a rare population that only represents 0.1% of total cells in the thymus organ, play an essential role in removing apoptotic thymocytes and maintaining thymus homeostasis. However, it remains unclear how thymic-resident macrophages can efficiently engulf apoptotic thymocytes. Therefore, we sorted the primary macrophages and performed metabolomics, RNA sequencing, and single-cell RNA sequencing analyses. Together with the in vitro studies, our work demonstrates the thymic-resident macrophages overcome continuous efferocytosis-induced oxidative stress by up-regulating the PPP, which highlights the central role of the PPP in maintaining thymic-resident macrophage and efferocytotic macrophage functions.During the research, I formed the hypothesis of PPP supporting the neutralization of oxidative stress in the thymic macrophages. With Dr. Chia-Lin Hsu’s guidance, we designed the proof-of-concept experiments, including the breakthrough of the challenging ex vivo thymic explant treatment. Furthermore, to achieve the complex multi-omics analysis of the metabolism network, I had learned R programming to accomplish the overall understanding of thymic macrophage metabolism. Together with flow cytometry, primary cell sorting, immunofluorescence staining, mitochondria content measurement, and cell culture experiments, we have put all the pieces together to prove our hypothesis. Not only did I learn bioinformatics skills and perform experiments, but I had also built a complete study from scratch. After three years of hard work in the laboratory, we have set up a milestone in investigating the rare thymic population.

• MSc Student

  • Jul 2018 - Aug 2020

  • Dean's Award of College of Life Science, National Yang-Ming University• Excellent Master Thesis Award, National Yang-Ming University• Honor member of Phi Tau Phi Honor Society• Dr. Hsu, Chialin's lab• Dissertation: https://hdl.handle.net/11296/7my26cTaipei, Taiwan



iGEM

• United States
• Biotechnology Research
• 100 - 200 Employee

• Leader, Presenter, Experimental group

  • Dec 2016 - Nov 2017

  • CGU_Taiwan, The International Genetically Engineered Machine Competition • Rewarded for the Bronze medal of the International Genetically Engineered Competition • Team leader of CGU_Taiwan (Chang Gung University) • Project: INKOFF: Preserve Fibers to Save Nature Reprocessing paper is very important for reducing deforestation, but the current manufacturing process causes chemical pollution and substantial paper fiber losses. After extensive discussions with paper-making experts, we have devised a plan to replace the chemicals used in traditional paper reprocessing and to increase recovery of paper fibers. In our model, the paper sheets undergo image analysis to determine which spots have ink printed on top and will be sprayed with Saccharomyces cerevisiae which has been engineered to express deinking enzymes upon light induction. Instead of treating all paper pulp, only the spots with ink is shone with light to induce localized deinking. This procedure can remove ink efficiently while protecting most of the paper fibers from being digested. This design extends the lifetime of the paper fibers and reduces wood-logging for making paper.



Chang Gung University

• Research Services
• 400 - 500 Employee

• Asthma mice model

  • Feb 2016 - Jan 2017

  • Protein expression of Thymic stromal lymphopoietin in OVA-sensitized mice model• 8 months• Professor Ming-lin Kou's lab• We are working on finding the potential target for AAV knockdown in asthma therapy by using OVA-sensitized mice model.

• Antibody drug engineering

  • Jul 2016 - Sep 2016

  • Development of a protease-activated antibody that reduces the side effects of Rheumatoid Arthritis therapy• 2 months (summer project)• Professor Tian-Lu Cheng's lab, Kaohsiung Medical University• We tried to lower the side effects of Remicade, Humira, and other mAb drugs by adding a protease-activable blockage on the epitopes.

• Cell cycle and the cell segregation

  • Sep 2015 - Feb 2016

  • The cell cycle and the cell segregation• 6 months• Professor Scott C. Schuyler's lab• We study the interaction of CDC20 and APC in cell cycle by using Saccharomyces cerevisiae.