Tamsen Polley
Anatomic Pathology Resident at Carlson College of Veterinary Medicine - Oregon State University- Claim this Profile
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Bio
Experience
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Carlson College of Veterinary Medicine - Oregon State University
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United States
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Veterinary Services
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1 - 100 Employee
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Anatomic Pathology Resident
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Jul 2021 - Present
Anatomic Pathology Resident and PhD Candidate Anatomic Pathology Resident and PhD Candidate
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Banfield Pet Hospital
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United States
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Veterinary Services
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700 & Above Employee
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Associate Veterinarian
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Jun 2020 - May 2021
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University of California, Davis
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United States
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Higher Education
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700 & Above Employee
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Doctoral Student
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Aug 2016 - Jun 2020
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Acting Lab Manager (Junior Research Specialist)
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Oct 2015 - Jul 2016
Hess Laboratory in the Animal Science Department.
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MSc Student
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Sep 2013 - Sep 2015
Completed a Masters of Science in Avian Sciences at the University of California Davis under Dr. Joanne Paul-Murphy. Thesis: "The welfare benefits of naturalistic feeding behavior in orange-winged Amazon parrots"
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UC Davis School of Veterinary Medicine
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United States
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Higher Education
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100 - 200 Employee
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Students Training in Advanced Research (STAR) Student
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May 2017 - Aug 2017
STAR summer project that was a collaboration between the Pesavento Lab at the University of California, Davis and the Beatty Lab at the University of Sydney, Australia. • Investigated the pathogenesis and etiologic role of a gammaherpesvirus in feline lymphoma • Aided in the design of a collaborative summer research project between two universities. • Advanced skills in tissue extraction, PCR, electrophoresis, gel imaging, histology, and in-situ hybridization. • Independently… Show more STAR summer project that was a collaboration between the Pesavento Lab at the University of California, Davis and the Beatty Lab at the University of Sydney, Australia. • Investigated the pathogenesis and etiologic role of a gammaherpesvirus in feline lymphoma • Aided in the design of a collaborative summer research project between two universities. • Advanced skills in tissue extraction, PCR, electrophoresis, gel imaging, histology, and in-situ hybridization. • Independently interpreted results and prepared a presentation for an end-of-project report and ACVP conference. Show less STAR summer project that was a collaboration between the Pesavento Lab at the University of California, Davis and the Beatty Lab at the University of Sydney, Australia. • Investigated the pathogenesis and etiologic role of a gammaherpesvirus in feline lymphoma • Aided in the design of a collaborative summer research project between two universities. • Advanced skills in tissue extraction, PCR, electrophoresis, gel imaging, histology, and in-situ hybridization. • Independently… Show more STAR summer project that was a collaboration between the Pesavento Lab at the University of California, Davis and the Beatty Lab at the University of Sydney, Australia. • Investigated the pathogenesis and etiologic role of a gammaherpesvirus in feline lymphoma • Aided in the design of a collaborative summer research project between two universities. • Advanced skills in tissue extraction, PCR, electrophoresis, gel imaging, histology, and in-situ hybridization. • Independently interpreted results and prepared a presentation for an end-of-project report and ACVP conference. Show less
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Scientific Illustrator
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Jan 2010 - Aug 2016
Illustrations that I have created for professional use, scientific publication, or educational purposes. Illustrations that I have created for professional use, scientific publication, or educational purposes.
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Oregon Health & Science University
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Hospitals and Health Care
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700 & Above Employee
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Research Assistant II
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Oct 2012 - Jul 2013
• Managed stocks of Leishmania lifestages & J774 macrophages (BSL II). • Researched NT1.1 3’UTR function in glucose transporters for possible drug targets (library construction and chromosomal integrations). • Utilized agarose gel electrophoresis, Southern/Western blot, PCR, electroporation, direct colony PCR, Renilla luciferase analysis, plasmid purification, RNA/DNA extraction, site-directed mutagenesis and transfections. • Aided in the construction of high throughput assays… Show more • Managed stocks of Leishmania lifestages & J774 macrophages (BSL II). • Researched NT1.1 3’UTR function in glucose transporters for possible drug targets (library construction and chromosomal integrations). • Utilized agarose gel electrophoresis, Southern/Western blot, PCR, electroporation, direct colony PCR, Renilla luciferase analysis, plasmid purification, RNA/DNA extraction, site-directed mutagenesis and transfections. • Aided in the construction of high throughput assays for drug development against both Leishmania spp. and Plasmodium falciparum. • Designed and implemented 96-well plate high throughput macrophage infection protocol for in vitro Leishmania spp. drug analysis. Show less • Managed stocks of Leishmania lifestages & J774 macrophages (BSL II). • Researched NT1.1 3’UTR function in glucose transporters for possible drug targets (library construction and chromosomal integrations). • Utilized agarose gel electrophoresis, Southern/Western blot, PCR, electroporation, direct colony PCR, Renilla luciferase analysis, plasmid purification, RNA/DNA extraction, site-directed mutagenesis and transfections. • Aided in the construction of high throughput assays… Show more • Managed stocks of Leishmania lifestages & J774 macrophages (BSL II). • Researched NT1.1 3’UTR function in glucose transporters for possible drug targets (library construction and chromosomal integrations). • Utilized agarose gel electrophoresis, Southern/Western blot, PCR, electroporation, direct colony PCR, Renilla luciferase analysis, plasmid purification, RNA/DNA extraction, site-directed mutagenesis and transfections. • Aided in the construction of high throughput assays for drug development against both Leishmania spp. and Plasmodium falciparum. • Designed and implemented 96-well plate high throughput macrophage infection protocol for in vitro Leishmania spp. drug analysis. Show less
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Oregon State University
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Higher Education
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700 & Above Employee
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Laboratory Technician/Researcher
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2010 - Jul 2012
• Thesis: supplemental analysis of Myxobolus squamalis based on small ribosomal subunit DNA sequencing & detection of M. squamalis in and around Leaburg and McKenzie Hatcheries. • Determining life cycles of M. insidiosus, M. squamalis, and M. cerebralis. • Recognition and collection of parasites from environment and organisms. • Extraction of DNA from environmental water/tissue samples for PCR use. • Design of new PCR assay for M. squamalis water detection.
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Laboratory Technician
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Sep 2009 - Jun 2010
• Managed multiple floors of hermaphroditic and self-fertilizing strains of inbred Biomphalarai glabrata snails. • Required high level of skill in sterile techniques due to risk of mixing strains. • Risk of infection: Schistosoma mansoni is listed as a biosafety level II.
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Oregon Health & Science University
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Hospitals and Health Care
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700 & Above Employee
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Research Assistant I
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Jun 2010 - Sep 2010
• Managed stocks of Leishmania mexicana promastigotes (biosafety level II). • Researched genes regulating glucose transporters in promastigotes. • Utilized agarose gel electrophoresis, electroporation, TOPO cloning, plasmid purification, DNA extraction, and transfections. • Completed and analyzed growth curves on L. mexicana mutants. • Managed stocks of Leishmania mexicana promastigotes (biosafety level II). • Researched genes regulating glucose transporters in promastigotes. • Utilized agarose gel electrophoresis, electroporation, TOPO cloning, plasmid purification, DNA extraction, and transfections. • Completed and analyzed growth curves on L. mexicana mutants.
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Portland State University
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United States
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Higher Education
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700 & Above Employee
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Laboratory Technician/Researcher
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Jul 2009 - Sep 2009
• Managed C. briggsea nematode stocks. • Completed fitness assays on varying mutants of C. briggsea. • Responsible for general lab clean-up and personal area. • Managed C. briggsea nematode stocks. • Completed fitness assays on varying mutants of C. briggsea. • Responsible for general lab clean-up and personal area.
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Oregon State University
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Higher Education
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700 & Above Employee
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Laboratory Technician/Researcher
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Sep 2008 - Jun 2009
• Isolated/archived mycobacteriophage species: OCGmgTP-EPE4-4. • Utilized DNA extraction, lysate purification (from agar plates), Nanodrop, electron microscopy, and restriction digest analysis. • Analyzed plaque morphologies and phage morphotypes. • Sequenced phage genome and analyzed it with Sequencher, gene called with Glimmer and GeneMark, and established a phamily in Phamerator. • Isolated/archived mycobacteriophage species: OCGmgTP-EPE4-4. • Utilized DNA extraction, lysate purification (from agar plates), Nanodrop, electron microscopy, and restriction digest analysis. • Analyzed plaque morphologies and phage morphotypes. • Sequenced phage genome and analyzed it with Sequencher, gene called with Glimmer and GeneMark, and established a phamily in Phamerator.
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Portland State University
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United States
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Higher Education
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700 & Above Employee
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Laboratory Technician/Researcher
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Jun 2007 - Sep 2007
• Responsible for general lab clean-up and bench. • Mutated “YkVM” gene unaided to meet specified requirements. • Supported PSU in the quest to reduce energy consumption by large plants. • Utilized PCR (error-prone), electrophoresis, DNA extraction, culturing cells. • Responsible for general lab clean-up and bench. • Mutated “YkVM” gene unaided to meet specified requirements. • Supported PSU in the quest to reduce energy consumption by large plants. • Utilized PCR (error-prone), electrophoresis, DNA extraction, culturing cells.
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Education
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University of California, Davis
Doctor of Veterinary Medicine (D.V.M.), Veterinary Pathology and Pathobiology -
University of California, Davis
Master of Science (M.S.), Avian Sciences -
Oregon State University
Honors Bachelor's Degree, Microbiology, Chemistry Minor