Experience

Biotheryx, Inc.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Scientist

  • Apr 2016 - Feb 2022

  In vitro evaluation of PHM and PROTAC molecules in primary blood cells and relevant cell lines using appropriate methodologies for establishing activity and potency in known targeted biological pathways as well as to evaluate affected pathways for the identification of novel druggable targets. In vitro evaluation of PHM and PROTAC molecules in primary blood cells and relevant cell lines using appropriate methodologies for establishing activity and potency in known targeted biological pathways as well as to evaluate affected pathways for the identification of novel druggable targets.



Celgene

• United States
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Scientist II

  • 2011 - 2015

  Inflammation Department • Utilized siRNA gene silencing and CRISPR/Cas9 gene knock-out methods for target validation • Utilized small molecule tool compounds for target perturbation • Seven I&I targets evaluated • Lead biologist for three targets pushed through to timely go/no-go decisions • Integral team member providing evaluation of multiple targets, one of which was nominated as a drug candidate

• Scientist I/II

  • 2002 - 2011

  Genomics Department Active participant and supervisor in providing gene array support for Biology groups at Celgene San Diego, San Francisco and Summit, New Jersey. • Genomics group supported > 50 experiments/year utilizing >1100 gene chips (GE,CNV)/year • Genomic assays conducted on a variety of sample sources including cultured cells, animal tissues, and clinical blood samples • Management and archiving of genomic experimental requests, sample information and data… Show more Genomics Department Active participant and supervisor in providing gene array support for Biology groups at Celgene San Diego, San Francisco and Summit, New Jersey. • Genomics group supported > 50 experiments/year utilizing >1100 gene chips (GE,CNV)/year • Genomic assays conducted on a variety of sample sources including cultured cells, animal tissues, and clinical blood samples • Management and archiving of genomic experimental requests, sample information and data files utilizing an in-house data base system • Investigational testing, implementation and recommendation for utilization of other genomic platforms such as HRM, CN and mutation qPCR assays



Signal Pharmaceuticals LLC

• United States
• Biotechnology Research

• Associate Scientist

  • 2000 - 2001

  Selective Estrogen Receptor Modulator (SERM) Group: • Characterized the structure, activity and relationship (SAR) of novel SERM alpha and beta compounds for non-proliferative (or anti-estrogenic) and anti-osteoporosis properties using mammary epithelial, ovarian and osteosarcoma cellular assays • Characterize SERM alpha and beta regulated gene transcripts by RNase protection assay • Maintain epithelial and osteosarcoma cell lines • Manage the SERM group tissue culture facility

• Senior Research Associate

  • 1999 - 2000

  Lead Discovery Group: • Characterized the structure, activity and relationship (SAR), along with the mechanism of action of small molecule inhibitors of NFkB, JNK, p38-2, and MEK-6 kinase pathways using radioactive in vitro biochemical assays • Generated dose response plots of compiled data and presented the determined IC50s values along with information regarding ATP competition at weekly group meetings

• Senior Research Associate

  • 1998 - 1999

  Estrogen Regulated Genes (ERG) Group • Characterized estrogen regulated genes from established vascular endothelial cell lines by RNA fingerprinting • Isolated and cloned estrogen regulated candidate gene • Identified estrogen regulated candidate genes by automated DNA sequencing (ABI Prism 377) • Validated estrogen regulated candidate genes by northern analysis, RNA protection assay (RPA) or in situ hybridization

• Research Associate

  • 1994 - 1998

  Neuroscience Department • Characterized human CNS and PNS cell lines for the presence of relevant m-RNAs using non- radioactive in situ hybridization (ISH) • Detected relevant m-RNAs in human fetal and rat fetal cryosections by ISH • Analyzed established human CNS and PNS cell lines for the presence of neuronal and non- neuronal proteins by immunocytochemistry and western blotting methods • Established and maintained human and rodent CNS cultures in defined… Show more Neuroscience Department • Characterized human CNS and PNS cell lines for the presence of relevant m-RNAs using non- radioactive in situ hybridization (ISH) • Detected relevant m-RNAs in human fetal and rat fetal cryosections by ISH • Analyzed established human CNS and PNS cell lines for the presence of neuronal and non- neuronal proteins by immunocytochemistry and western blotting methods • Established and maintained human and rodent CNS cultures in defined media • Managed the neuroscience group tissue culture facility



NVRI

• Reseach Associate

  • 1990 - 1991