Experience

EpiVario, Inc.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist

  • Jul 2022 - Present



Houston Methodist

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Postdoctoral Fellow

  • Apr 2017 - Mar 2023

  Study of myelin biology in regeneration using viral reporter systems Laboratory management: - Implemented organizational protocols for laboratory management and inventory - Organized and oversaw the physical move of the laboratory to a new space - Mentored and trained several staff members and undergraduate students - Launched and co-lead the inaugural year of the Center for Neuroregeneration Lecture series (email speakers, organize schedule and host speakers) - Organize and present research in front of peers (lab meetings) and at international conference (Myelin Gordon Research Conference 2018) Animal work: - Write and administer IACUC protocols - Perform small rodent surgeries (laminectomy, spinal injection, Burr hole and stereotactic injection in brain, pup injection into brain and spinal cord) and associated post-operational care as needed - Assayed mice on several behavior assays, including elevated-plus maze, Y-maze, Novel object recognition, rotarod, digigait, marble burying, puzzle box and Barnes maze. - Imaged mice on 9.4T MRI system - Colony management and genotyping Use confocal microscopy and imaging softwares (MBF stereoInvestigator, Neurolucida) to image and quantify Central Nervous system tissue (myelin, glial cells, axons) Developed and maintain primary cultures of brains and spinal cords from mini pigs for adult neural stem cell cultures (3D culture in Matrigel, differentiation protocols, transformation into glioblastoma, in vitro tumor assays) Cloned and generated retroviral and lentiviral constructs Show less



Baylor College of Medicine

• United States
• Higher Education
• 700 & Above Employee

• Ph.D. Graduate Student

  • Aug 2009 - Jan 2017

  - Designed and led an independent temporal profiling screen to identify novel genes in gliogenesis - Performed FACS on embryonic cells to identified novel genes expressed in astrocyte during mouse development - Collaborated on various projects with different groups on different topics at Baylor College of Medicine (Dr. Arenkiel, Dr. Borowiak) and at UCSF (Dr. Rowitch). - Performed a variety of molecular biology techniques (DNA and RNA extraction, RT- and Q-PCR, cloning, primer design, Co-immunoprecipiration, chromatin-immunoprecipitation) - Conducted demyelination injury in mice (lysolecithin in adult and photothrombotic stroke in pups), hypoxic injury on pups, chick in-ovo electroporation - Some experience with In Utero Electroporation using piggy-bag and CRISPR-Cas constructs - Presented posters and talks at Baylor College of Medicine retreats and international conference (American Society for Neurochemistry, 2014) Show less



Baylor College of Medicine - Human Genome Sequencing Center

• Houston, Texas Area

• Research Assistant

  • Feb 2008 - Jul 2009

  - Responsible for the inventory and ordering for Solexa/Illumina sequencing - Troubleshot and maintained Solexa/Illumina instruments - Prepared samples for Next. Gen. Sequencing on the Solexa/Illumina - Involved with the running of the SOLiD ABI instruments - Responsible for the inventory and ordering for Solexa/Illumina sequencing - Troubleshot and maintained Solexa/Illumina instruments - Prepared samples for Next. Gen. Sequencing on the Solexa/Illumina - Involved with the running of the SOLiD ABI instruments



Purdue University

• United States
• Higher Education
• 700 & Above Employee

• M.Sc. Graduate Student

  • Aug 2005 - Dec 2007

  Study of apical polarity of normal and tumor Human breast epithelial cells using in vitro 3D culture - 3D culture of breast epithelial cells - Various in vitro assays (proliferation, differentiation, drug survival assays, ELISA) - Protein and RNA work - Immunohistochemistry on Human Mammary Tissue Cell Culture room management and inventory Study of apical polarity of normal and tumor Human breast epithelial cells using in vitro 3D culture - 3D culture of breast epithelial cells - Various in vitro assays (proliferation, differentiation, drug survival assays, ELISA) - Protein and RNA work - Immunohistochemistry on Human Mammary Tissue Cell Culture room management and inventory



Institut Pasteur

• France
• Research Services
• 700 & Above Employee

• Internship Trainee

  • May 2002 - Jun 2002

  A 10 weeks internship at the Pasteur Institute in the bacterial membrane unit. A 10 weeks internship at the Pasteur Institute in the bacterial membrane unit.