Experience

Tokyo Institute of Technology

• Japan
• Higher Education
• 500 - 600 Employee

• PhD student

  • Apr 2021 - Apr 2022

  Here are my thesis topics: -Elucidation of GroE function at low temperature -A challenge to conventional protein science: Protein folding analysis affected by identical mutations After a year of thesis I was struck down by an illness which forced me to stop my thesis to follow my treatment. One year after my Treatment (2023) I was able to take control of my life. Here are my thesis topics: -Elucidation of GroE function at low temperature -A challenge to conventional protein science: Protein folding analysis affected by identical mutations After a year of thesis I was struck down by an illness which forced me to stop my thesis to follow my treatment. One year after my Treatment (2023) I was able to take control of my life.



The University of Tokyo

• Japan
• Research Services
• 700 & Above Employee

• Laboratory Technician - Internship supervisor

  • Sep 2020 - Apr 2021

  At the end of my internship (September 2020) I stayed in the Ui-Tei laboratory until I could start my thesis (April 2021). During this period, I was able to complete the part of the project that had been assigned to me. Following this, with the members of the laboratory, we filed a patent. In early July 2021, my results will be presented at a conference. During the last 4 months, in addition to assisting the work in the laboratory, I have also been the internship supervisor of an… Show more At the end of my internship (September 2020) I stayed in the Ui-Tei laboratory until I could start my thesis (April 2021). During this period, I was able to complete the part of the project that had been assigned to me. Following this, with the members of the laboratory, we filed a patent. In early July 2021, my results will be presented at a conference. During the last 4 months, in addition to assisting the work in the laboratory, I have also been the internship supervisor of an American student. I passed my knowledge on to him and supervised his project.

• Engineer internship

  • Mar 2020 - Sep 2020

  Many diseases can occur as a result of a quantitative or qualitative modification of our genes. This is the case with cancers that can be caused by a change or damage that can occur in the genes. For a long time, RNA appeared to be simple intermediaries between DNA and proteins. But a whole new world of small, non-coding RNAs has been discovered since the late 1990s: Small interfering RNAs, such as siRNAs, are small RNAs that can specifically bind to a sequence of messenger RNAs to prevent… Show more Many diseases can occur as a result of a quantitative or qualitative modification of our genes. This is the case with cancers that can be caused by a change or damage that can occur in the genes. For a long time, RNA appeared to be simple intermediaries between DNA and proteins. But a whole new world of small, non-coding RNAs has been discovered since the late 1990s: Small interfering RNAs, such as siRNAs, are small RNAs that can specifically bind to a sequence of messenger RNAs to prevent gene expression. They have a bright future today, especially in therapeutic tools. Like patisiran, which was the first drug representing this class of substances to receive marketing authorization in August 2018, new research is being carried out with the aim of producing other drugs designed from siRNA. In the laboratory of Mrs. UI-TEI, a 30ene of new candidate genes are studied. I have been asked to study 16 of these genes whose diseases caused by mutations are mainly linked to cancer. The efficiency of an elaborated siRNA is determined according to its ability to inhibit the mutated gene without disturbing the wild-type version of the same gene. All of this is evaluated using the luciferase assay. The aim is to develop siRNAs which cleave mutated genes without disturbing their wild versions and other endogenous genes. Final score : 17/20



École de Biologie Industrielle

• France
• Higher Education
• 100 - 200 Employee

• Research and Innovation in Advanced Biology

  • Sep 2019 - Feb 2020

  Wastewaters, in textile industry, are heavily contaminated with many chemicals, including dyes. Biological treatments are promising and are based on the use of microorganisms and certain enzymes such as laccases. The long-term goal of this research is to produce recombinant laccases in order to develop an enzymatic bioreactor to treat these effluents. Chaetomium globosum (Cgo) and Bacills subtilis (CotA) laccases have been studied for this purpose. The cloning and expression of the genes of… Show more Wastewaters, in textile industry, are heavily contaminated with many chemicals, including dyes. Biological treatments are promising and are based on the use of microorganisms and certain enzymes such as laccases. The long-term goal of this research is to produce recombinant laccases in order to develop an enzymatic bioreactor to treat these effluents. Chaetomium globosum (Cgo) and Bacills subtilis (CotA) laccases have been studied for this purpose. The cloning and expression of the genes of these two laccases was carried out using pNCMO2 plasmid of Brevibacillus expression system. Protein expression was analyzed by SDS-PAGE. The structure analysis of Cgo laccase with Phyre2 software suggest that its oriented immobilization can be carried out on alumina without impacting its enzymatic activity. Key words: Laccase, Chaetomium globosum, Brevibacillus expression system, wastewater, immobilization.

• University project

  • Sep 2018 - Sep 2019

  This project was born as part of an Innovation and Business Creation project focused on food and more particularly fruit. The interest is to solve a major problem which is the lack of fruit and vegetable consumption among the elderly, who represent nearly 20% of the population. To meet these expectations, we have created a product called Soupra'Bon: an old-fashioned vegetable soup, 100% natural and enriched with a complex of superfruits. These super fruits contain more vitamins or other… Show more This project was born as part of an Innovation and Business Creation project focused on food and more particularly fruit. The interest is to solve a major problem which is the lack of fruit and vegetable consumption among the elderly, who represent nearly 20% of the population. To meet these expectations, we have created a product called Soupra'Bon: an old-fashioned vegetable soup, 100% natural and enriched with a complex of superfruits. These super fruits contain more vitamins or other elements than other fruits, which helps in improving health like geriatric symptoms. Beyond our B to B marketing (retirement homes, EHPAD ...), we do not forget the B to C sale for independent elderly people, nor other future prospects such as soup in jelly using the concept of Finger Food to restore the esteem and place of the subject to people suffering from mechanical disorders and who must be assisted. Key words: Alimentary, Soup, Super fruits, Old people, Health

• Assistanat

  • Jan 2018 - Jun 2019

  Optimisation de la production de protéase TEV à l'aide de différentes souches d'Escherichia coli et de différentes techniques de purifications et d'extractions La TEV protéase (Tobacco Etch Virus) est une cystéine protéase hautement spécifique à la séquence du Tobacco Etch Virus. L'une des principales utilisations de cette protéine est l'élimination des marqueurs d'affinité des protéines de fusion recombinantes purifiées. La raison de l'utilisation de la protéase TEV comme outil… Show more Optimisation de la production de protéase TEV à l'aide de différentes souches d'Escherichia coli et de différentes techniques de purifications et d'extractions La TEV protéase (Tobacco Etch Virus) est une cystéine protéase hautement spécifique à la séquence du Tobacco Etch Virus. L'une des principales utilisations de cette protéine est l'élimination des marqueurs d'affinité des protéines de fusion recombinantes purifiées. La raison de l'utilisation de la protéase TEV comme outil biochimique est sa spécificité de séquence élevée. Cette spécificité permet le clivage contrôlé des protéines lorsque la séquence de préférence est insérée dans des boucles flexibles. Cela le rend également relativement non toxique in vivo car la séquence reconnue se produit rarement dans les protéines. L'objectif est de trouver le meilleur paramètre pour optimiser la production de protéase TEV en utilisant différentes souches d'Escherichia coli (BL21pLys, pRare, BL21 DsbC et KRX) puis d'utiliser différentes techniques de purifications (lyse / sonification) et d'extractions (billes de nickel / batch).



Tokyo Institute of Technology

• Japan
• Higher Education
• 500 - 600 Employee

• Engineer internship

  • Jun 2018 - Jul 2018

  An important goal in chaperone biology is to identify a subset of chaperonin GroEL/GroES substrates. This helps to understand the versatile roles of chaperones in the cell. The identification of the substrates of this chaperone was particularly studied at 37° C. But many chaperones are expressed in response to changes in temperature. Before my arrival, an experiment was conducted on the chaperone of interest with the temperature variation: a study was conducted at 18° C. At this temperature,… Show more An important goal in chaperone biology is to identify a subset of chaperonin GroEL/GroES substrates. This helps to understand the versatile roles of chaperones in the cell. The identification of the substrates of this chaperone was particularly studied at 37° C. But many chaperones are expressed in response to changes in temperature. Before my arrival, an experiment was conducted on the chaperone of interest with the temperature variation: a study was conducted at 18° C. At this temperature, a substrate was identified as a pertinent substrate: Suc D. During this internship, it has been proposed to clone the gene of suc D and his partner: suc C in the pET21c vectors with the presence of the poly-histidine tag. The aim of this cloning is to carry out an affinity chromatography and to purify it as a complex, Suc D and Suc C. We have, subsequently, evaluated the enzymatic activity and the folding mechanism of the enzyme. During this study, we varied various parameters including the temperature and the concentration of our protein. Keywords: Chaperonin GroEL/GroES; Protein Suc D/ Suc C; enzymatic activity; folding capacity; percentage of aggregation. Final score : 18.07/20 Show less An important goal in chaperone biology is to identify a subset of chaperonin GroEL/GroES substrates. This helps to understand the versatile roles of chaperones in the cell. The identification of the substrates of this chaperone was particularly studied at 37° C. But many chaperones are expressed in response to changes in temperature. Before my arrival, an experiment was conducted on the chaperone of interest with the temperature variation: a study was conducted at 18° C. At this temperature,… Show more An important goal in chaperone biology is to identify a subset of chaperonin GroEL/GroES substrates. This helps to understand the versatile roles of chaperones in the cell. The identification of the substrates of this chaperone was particularly studied at 37° C. But many chaperones are expressed in response to changes in temperature. Before my arrival, an experiment was conducted on the chaperone of interest with the temperature variation: a study was conducted at 18° C. At this temperature, a substrate was identified as a pertinent substrate: Suc D. During this internship, it has been proposed to clone the gene of suc D and his partner: suc C in the pET21c vectors with the presence of the poly-histidine tag. The aim of this cloning is to carry out an affinity chromatography and to purify it as a complex, Suc D and Suc C. We have, subsequently, evaluated the enzymatic activity and the folding mechanism of the enzyme. During this study, we varied various parameters including the temperature and the concentration of our protein. Keywords: Chaperonin GroEL/GroES; Protein Suc D/ Suc C; enzymatic activity; folding capacity; percentage of aggregation. Final score : 18.07/20 Show less



French Agency for Food, Environmental and Occupational Health & Safety (ANSES)

• France
• Government Administration
• 700 & Above Employee

• Student internship

  • Nov 2016 - Dec 2016

  Internship carried out in animal health laboratory Validation of interactions between foot-and-mouth disease virus proteins and bovine proteins. Foot-and-mouth disease is a highly contagious viral animal disease affecting domestic and wild artiodactyls (mainly cattle, pigs, sheep, goats) and whose socio-economic impact in the event of an epizootic is considerable. The causative agent, foot-and-mouth disease virus, is a virus of the genus Aphtovirus of the family Picornaviridae. This… Show more Internship carried out in animal health laboratory Validation of interactions between foot-and-mouth disease virus proteins and bovine proteins. Foot-and-mouth disease is a highly contagious viral animal disease affecting domestic and wild artiodactyls (mainly cattle, pigs, sheep, goats) and whose socio-economic impact in the event of an epizootic is considerable. The causative agent, foot-and-mouth disease virus, is a virus of the genus Aphtovirus of the family Picornaviridae. This virus is characterized by significant genetic and antigenic variability and has a worldwide distribution. The mechanisms for establishing and maintaining persistence are not clearly understood to date, but work based on in vitro approaches indicates co-evolution of FMDV and host cells during persistence. There is also a lack of specific knowledge regarding the innate antiviral response in ruminants and its possible role in establishing and / or maintaining persistent FMD virus infections. During this internship, I was asked to study whether the mutations that appeared, during persistence, in certain sequences have functional consequences compared to the homologous sequences of the virus. The data from this study provide a better understanding of the pathogenesis of foot-and-mouth disease by identifying the cell signaling pathways modulated during infection (acute or persistent) and could therefore contribute to the development of better strategies for the control of foot-and-mouth disease. Key words: Foot and Mouth Disease Virus, virus persistence, site-directed mutagenesis, luciferase assay, cell culture. Final score: 20/20 Show less Internship carried out in animal health laboratory Validation of interactions between foot-and-mouth disease virus proteins and bovine proteins. Foot-and-mouth disease is a highly contagious viral animal disease affecting domestic and wild artiodactyls (mainly cattle, pigs, sheep, goats) and whose socio-economic impact in the event of an epizootic is considerable. The causative agent, foot-and-mouth disease virus, is a virus of the genus Aphtovirus of the family Picornaviridae. This… Show more Internship carried out in animal health laboratory Validation of interactions between foot-and-mouth disease virus proteins and bovine proteins. Foot-and-mouth disease is a highly contagious viral animal disease affecting domestic and wild artiodactyls (mainly cattle, pigs, sheep, goats) and whose socio-economic impact in the event of an epizootic is considerable. The causative agent, foot-and-mouth disease virus, is a virus of the genus Aphtovirus of the family Picornaviridae. This virus is characterized by significant genetic and antigenic variability and has a worldwide distribution. The mechanisms for establishing and maintaining persistence are not clearly understood to date, but work based on in vitro approaches indicates co-evolution of FMDV and host cells during persistence. There is also a lack of specific knowledge regarding the innate antiviral response in ruminants and its possible role in establishing and / or maintaining persistent FMD virus infections. During this internship, I was asked to study whether the mutations that appeared, during persistence, in certain sequences have functional consequences compared to the homologous sequences of the virus. The data from this study provide a better understanding of the pathogenesis of foot-and-mouth disease by identifying the cell signaling pathways modulated during infection (acute or persistent) and could therefore contribute to the development of better strategies for the control of foot-and-mouth disease. Key words: Foot and Mouth Disease Virus, virus persistence, site-directed mutagenesis, luciferase assay, cell culture. Final score: 20/20 Show less



French Agency for Food, Environmental and Occupational Health & Safety (ANSES)

• France
• Government Administration
• 700 & Above Employee

• Student internship

  • Jul 2015 - Aug 2015

  Internship carried out in animal health laboratory Molecular interactions between the foot-and-mouth disease virus and the bovine host cell in the context of a persistent infection Internship carried out in animal health laboratory Molecular interactions between the foot-and-mouth disease virus and the bovine host cell in the context of a persistent infection