Luca Boschian
PhD student at University of Kiel- Claim this Profile
Click to upgrade to our gold package
for the full feature experience.
-
Italiano Native or bilingual proficiency
-
Inglese Full professional proficiency
-
Tedesco Professional working proficiency
Topline Score
Bio
Experience
-
Kiel University
-
Germany
-
Research Services
-
700 & Above Employee
-
PhD student
-
Jan 2012 - Present
Il principale obiettivo del mio lavoro è stato l'identificazione delle funzioni della proteina WHIRLY1 in orzo. A tal fine ho usato differenti metodi di biochimica, spaziando da metodi perparativi (isolazione di protoplasti, frazionamento cellulare su gradiente, isolazione di organelli) all'elettroforesi su gel di acrilammide (nativi e denaturanti, compresa IEF) a metodi di purificazione proteica (pre-frazionamento con ammonio solfato, gel filtration e cromatografia ad affinità). Ho anche avuto esperienza con differenti strumenti per la misura di parametri fisiologici (ImagingPAM, DualPAM, spettroscopia a bassa temperatura). Show less
-
-
-
NPZ
-
Kiel, Germania
-
Early Stage Researcher (ESR)
-
Jan 2012 - Nov 2014
Early Stage Researcher in the Marie Curie Fellowship program for the Initial Training Network (ITN) CropLife Early Stage Researcher in the Marie Curie Fellowship program for the Initial Training Network (ITN) CropLife
-
-
-
Università degli Studi di Padova
-
Italy
-
Higher Education
-
700 & Above Employee
-
Master thesis internship
-
Jan 2011 - Sep 2011
During my internship I learned the following tecniques: maintainment of cell coltures in sterility conditions, DNA and RNA extraction from calli and plants, PCR and RT-PCR, histochemical and fluorimetric GUS assay, cell death assay using Evans blue dye During my internship I learned the following tecniques: maintainment of cell coltures in sterility conditions, DNA and RNA extraction from calli and plants, PCR and RT-PCR, histochemical and fluorimetric GUS assay, cell death assay using Evans blue dye
-
-
-
Università degli Studi di Padova
-
Italy
-
Higher Education
-
700 & Above Employee
-
Bachelor thesis internship
-
Apr 2010 - Jul 2010
During my internship I learned the following tecniques: extraction and quantification of photosynthetic pigments, DNA extraction from algal tissues, quantification of heavy-metal uptake using atomic absorption spectrophotometry, HPLC, preparation of electron microscopy samples During my internship I learned the following tecniques: extraction and quantification of photosynthetic pigments, DNA extraction from algal tissues, quantification of heavy-metal uptake using atomic absorption spectrophotometry, HPLC, preparation of electron microscopy samples
-
-
Education
-
Università degli Studi di Padova
Master Degree, Evolutionary Biology -
Università degli Studi di Padova
Bachelor Degree, Biology