Nat Graham

Lead, Molecular Biology at Pairwise
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Contact Information
us****@****om
(386) 825-5501
Location
Durham, North Carolina, United States, US

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Experience

    • United States
    • Food and Beverage Manufacturing
    • 100 - 200 Employee
    • Lead, Molecular Biology
      • May 2021 - Present

    • Molecular Biology Scientist
      • Dec 2018 - May 2021

    • United States
    • Higher Education
    • 700 & Above Employee
    • Postdoctoral Associate
      • Aug 2017 - Dec 2018

    • United Kingdom
    • Biotechnology Research
    • 1 - 100 Employee
    • Contributing Author
      • May 2015 - Nov 2018

      Author of short publications designed to help other scientists both in and out of the lab. http://bitesizebio.com/profile/nathanielgraham/ Author of short publications designed to help other scientists both in and out of the lab. http://bitesizebio.com/profile/nathanielgraham/

    • United States
    • Higher Education
    • 700 & Above Employee
    • Graduate Researcher
      • Aug 2011 - Aug 2017

      Research Interests: Improving plant biotechnology through the use of minichromosomes and site-specific recombinases.For the past five years I have focused on methods to improve the maize engineering process. My first project focuses on the production of minichromosomes, small chromosomes containing a transgene and a method of selection. The project focuses on utilizing binary bacterial artificial chromosome (BiBAC) vectors to create mini chromosomes containing multiple transgress. The ultimate goal is to be able to produce minichromosomes that contain whole gene pathways, which could then be easily transferred between different maize lines. Another focus of my research is utilizing recombinases to be able to insert transgenes at specific locations. Utilizing the recombinase phiC31, inserted transgenes could be inserted into previously inserted recombination sites, eliminating the need to screen transgenic lines for optimal insertions. Lastly, I am attempting to improve the standard maize transformation and regeneration protocol by reviewing industry patents. While industry protocols are not usually published in traditional journals, it is known in the field that industry scientists are constantly working to improve the technique. By reviewing published patents, I am able to deduce the improvements they have made. Research Skills:Molecular techniques including: southern hybridization, fluorescent in-situ hybridization, cloning, PCR, Experimental designMaize regeneration and transformationSoft Skill Training Program: 1.) Selected to participate in a 4 month training program, including a personal coach, Preparing Tomorrow's Leaders for Science (PTLS). The course, provided for a small subset of 14 students at MU, was designed to provide critical soft skills that are otherwise not provided.2.) Selected to participate in a second 4 month training, advanced PTLS, designed to further develop the soft skills provided in the first PTLS course.

    • United States
    • Farming
    • 700 & Above Employee
    • Regulatory Affairs Intern
      • May 2011 - Jul 2011

      Maintained previously created response databaseConsolidated internal product websites to a central location Maintained previously created response databaseConsolidated internal product websites to a central location

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Researcher for Dr. Stephen Hudman
      • Jan 2009 - May 2011

      Assisted with aging and sexing fish species Assisted with aging and sexing fish species

    • United States
    • Farming
    • 700 & Above Employee
    • Regulatory Affairs Intern
      • May 2010 - Jul 2010

      Created a regulator response databaseCreated a database of references for future submissionsProofread product submission documents Created a regulator response databaseCreated a database of references for future submissionsProofread product submission documents

Education

  • University of Missouri-Columbia
    Doctor of Philosophy (Ph.D.), Biology, General
    2011 - 2017
  • Truman State University
    Bachelor of Science (B.S.), Biology
    2007 - 2011

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