Experience

Eurofins Biopharma Product Testing (France)

• France
• Pharmaceutical Manufacturing
• 100 - 200 Employee

• Quotation specialist

  • Apr 2019 - Present

  I guide customers by qualifying their requests and making the link with the laboratories (QC microbiology pharma, biochemistry analyzes of biologics and pharmaceutical gas and water utilities). I have a role of interface between the customers and the laboratory which requires a double hat: technical knowledge of the tests to be carried out and a customer orientation to satisfy their needs, to know how to guide them. The creation of quotes via the company's software (CRM, specific software for quotes and analyses), are real roadmaps for the laboratory. This position therefore requires a 360° vision: customer needs, analyzes to be implemented, impact of the estimate at the laboratory level and involvement in the sale and the results of the company. As Eurofins is an international company, interactions with other sites abroad are also frequent. Show less



COVALAB

• France
• Biotechnology
• 1 - 100 Employee

• Laboratory technician

  • Oct 2017 - Present

  One-year replacement on a laboratory technician position. Antibody purifications from ascites or cell supernatants, ELISA analysis. Participation in the life of a small business: product manufacturing, customer shipments. Immunogen preparations. One-year replacement on a laboratory technician position. Antibody purifications from ascites or cell supernatants, ELISA analysis. Participation in the life of a small business: product manufacturing, customer shipments. Immunogen preparations.



Sanofi

• France
• Pharmaceutical Manufacturing
• 700 & Above Employee

• DSP laboratory technician - R&D purification

  • Sep 2016 - Feb 2017

  6-month interim position. Analytical support to the purification platform for the development of candidate vaccines. Technics : o-acetyl, protein, amine, thiol assays. HPSEC analysis. Chemical conjugation technician, HPLC analyzes (AKTA Pure, gel filtration). 6-month interim position. Analytical support to the purification platform for the development of candidate vaccines. Technics : o-acetyl, protein, amine, thiol assays. HPSEC analysis. Chemical conjugation technician, HPLC analyzes (AKTA Pure, gel filtration).



Thermo Fisher Scientific

• United States
• Biotechnology Research
• 700 & Above Employee

• Technical support

  • Nov 2015 - Mar 2016

  Maternity leave replacement. Technical support to customers in France and occasionally Italy by e-mail and telephone. SAP, ISO standards, customer relations, attentiveness and speed of response to deadlines for large industrial companies (Bigard, Eurofins, Sanofi, etc.) or hospital staff. Maternity leave replacement. Technical support to customers in France and occasionally Italy by e-mail and telephone. SAP, ISO standards, customer relations, attentiveness and speed of response to deadlines for large industrial companies (Bigard, Eurofins, Sanofi, etc.) or hospital staff.



Institut Curie

• France
• Hospitals and Health Care
• 700 & Above Employee

• Engineer in Antibodies production and purification at Institut Curie – Plateforme Tab-IP -Paris

  • Sep 2012 - Mar 2015

  Projects: Production of monoclonal antibodies and VHHs to use as cancer molecular markers Techniques used : FPLC (AKTA Pure : affinity, desalting, SEC ; scouting and method queue in order to purify 8 VHHs in a row)) , bacterial production (BL21/DE3), eukaryote production (HEK293). Analysis of the purified antibodies by SDS-PAGE, Western blot, FACS and ELISA. Determination of the binding partner by ImmunoPrecipitation and Mass Spectrometrie. Cloning of the DNA sequence in different expression vectors by cut-and-paste or PCR. Show less



San Raffaele Biomedical Science Park

• Hospitals and Health Care
• 1 - 100 Employee

• Research assistant

  • Feb 2007 - Dec 2011

  Projects: Study of RACK1 conditional knock out mice. Production of the eucaryotic Initiation Factor 6 (eIF6) in insects cells and purification; Technics used : molecular biology, tissue culture, Baculovirus ingeneering, animal handling, work with mice embryos, stem cells, lentivirus and adenovirus, RNA purification and Northern blot, polysomes profiles, western blot, multiplex PCR, RT-PCR Supervising of phD students, writting of manuscipts, developpment of projects. Projects: Study of RACK1 conditional knock out mice. Production of the eucaryotic Initiation Factor 6 (eIF6) in insects cells and purification; Technics used : molecular biology, tissue culture, Baculovirus ingeneering, animal handling, work with mice embryos, stem cells, lentivirus and adenovirus, RNA purification and Northern blot, polysomes profiles, western blot, multiplex PCR, RT-PCR Supervising of phD students, writting of manuscipts, developpment of projects.



Sanofi

• France
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Research assistant

  • May 2006 - Sep 2006

  Technical assistance in developping an HTS using yeast double hybrid system. Technics used : Yeast culture, molecular biology. Technical assistance in developping an HTS using yeast double hybrid system. Technics used : Yeast culture, molecular biology.



INSERM

• France
• Research Services
• 700 & Above Employee

• Study ingineer

  • Sep 2004 - Oct 2005

  Technical support to post-docs in producing protein in bacteria. First experience with HPLC device : AKTA Purifier (GE). Tissue culture, protein expression in insect cells, HPLC. Technical support to post-docs in producing protein in bacteria. First experience with HPLC device : AKTA Purifier (GE). Tissue culture, protein expression in insect cells, HPLC.



University of Dundee

• United Kingdom
• Research Services
• 700 & Above Employee

• Research assistant

  • Sep 2001 - Jun 2004

  Study of the role of mammalian Target Of Rapamycin (mRNA regulator) on cell growth and proliferation. Leading of my onw projects, work in collaboration with Astra Zeneca. Technics: tissue culture, Western Blot, production and purification of proteins and antibodies, use of radioactifs , molecular biology. Study of the role of mammalian Target Of Rapamycin (mRNA regulator) on cell growth and proliferation. Leading of my onw projects, work in collaboration with Astra Zeneca. Technics: tissue culture, Western Blot, production and purification of proteins and antibodies, use of radioactifs , molecular biology.