Safa Chogule
Biofilm Scientist at BluTest Laboratories Ltd- Claim this Profile
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Experience
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BluTest Laboratories Ltd
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United Kingdom
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Biotechnology Research
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1 - 100 Employee
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Biofilm Scientist
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Apr 2022 - Present
I plan and develop experiment protocols in biofilm testing for aerobic, anaerobic and fungal microorganisms. Conducting research and development work with biofilms to validate different complex biofilm models.I am responsible for data analysis and visualization, writing conclusive reports and suggesting recommendations and feedbacks. I use various different microbiological assays such as multi-species biofilm testing, MIC/MBC testing, zone of inhibition, regrowth assays and time kill assays… Show more I plan and develop experiment protocols in biofilm testing for aerobic, anaerobic and fungal microorganisms. Conducting research and development work with biofilms to validate different complex biofilm models.I am responsible for data analysis and visualization, writing conclusive reports and suggesting recommendations and feedbacks. I use various different microbiological assays such as multi-species biofilm testing, MIC/MBC testing, zone of inhibition, regrowth assays and time kill assays along with different molecular assays such as DNA/RNA extractions, qPCR and ELISA. Working in a laboratory with Good Laboratory Practice (GLP), hence consistently with safety protocols, producing data that is accurate, compliant with regulatory standards and aware of COSHH forms. Show less I plan and develop experiment protocols in biofilm testing for aerobic, anaerobic and fungal microorganisms. Conducting research and development work with biofilms to validate different complex biofilm models.I am responsible for data analysis and visualization, writing conclusive reports and suggesting recommendations and feedbacks. I use various different microbiological assays such as multi-species biofilm testing, MIC/MBC testing, zone of inhibition, regrowth assays and time kill assays… Show more I plan and develop experiment protocols in biofilm testing for aerobic, anaerobic and fungal microorganisms. Conducting research and development work with biofilms to validate different complex biofilm models.I am responsible for data analysis and visualization, writing conclusive reports and suggesting recommendations and feedbacks. I use various different microbiological assays such as multi-species biofilm testing, MIC/MBC testing, zone of inhibition, regrowth assays and time kill assays along with different molecular assays such as DNA/RNA extractions, qPCR and ELISA. Working in a laboratory with Good Laboratory Practice (GLP), hence consistently with safety protocols, producing data that is accurate, compliant with regulatory standards and aware of COSHH forms. Show less
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University of Glasgow, College of Medical, Veterinary and Life Sciences
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United Kingdom
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100 - 200 Employee
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Research Assistant
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Nov 2021 - Apr 2022
Worked on multiple different projects with different corporations: I conducted testing on different bacterial strains against different actives to determine their planktonic and sessile MIC along with time-kill assays, growth curves, checkerboard assay and Miles and Misra to determine treatment of biofilms. Furthermore, carried out testings using hydrogel models. Gained hands on experience with human cell lines, cell stimulation, tissue fixing, permeabilization and blocking, DNA/RNA… Show more Worked on multiple different projects with different corporations: I conducted testing on different bacterial strains against different actives to determine their planktonic and sessile MIC along with time-kill assays, growth curves, checkerboard assay and Miles and Misra to determine treatment of biofilms. Furthermore, carried out testings using hydrogel models. Gained hands on experience with human cell lines, cell stimulation, tissue fixing, permeabilization and blocking, DNA/RNA extraction, cDNA synthesis, qPCR and human CXCL-8/IL-8 ELISA. Graduate teaching assistant: Trained and worked as a Graduate teaching assistant for the MSc students during theoretical and lab classes teaching statistical analysis, pipetting techniques, preparation of media, DNA extractions, ELISA, crystal violet assay and XTT assay. Show less Worked on multiple different projects with different corporations: I conducted testing on different bacterial strains against different actives to determine their planktonic and sessile MIC along with time-kill assays, growth curves, checkerboard assay and Miles and Misra to determine treatment of biofilms. Furthermore, carried out testings using hydrogel models. Gained hands on experience with human cell lines, cell stimulation, tissue fixing, permeabilization and blocking, DNA/RNA… Show more Worked on multiple different projects with different corporations: I conducted testing on different bacterial strains against different actives to determine their planktonic and sessile MIC along with time-kill assays, growth curves, checkerboard assay and Miles and Misra to determine treatment of biofilms. Furthermore, carried out testings using hydrogel models. Gained hands on experience with human cell lines, cell stimulation, tissue fixing, permeabilization and blocking, DNA/RNA extraction, cDNA synthesis, qPCR and human CXCL-8/IL-8 ELISA. Graduate teaching assistant: Trained and worked as a Graduate teaching assistant for the MSc students during theoretical and lab classes teaching statistical analysis, pipetting techniques, preparation of media, DNA extractions, ELISA, crystal violet assay and XTT assay. Show less
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University of Glasgow
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United Kingdom
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Higher Education
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700 & Above Employee
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Research Technician
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Sep 2021 - Nov 2021
I conducted growth promotion testing on bacterial and fungal strains including rehydrating organisms and maintaining stock cultures. Managed day to day running of the lab such as preparation and maintenance of cultures and medias, standardization of bacterial (anaerobic and aerobic) and fungal species for different experiments, MIC testing, biomass quantification, bacterial quantification and Alamar blue assay. I have performed molecular biology scientific research using methods such as… Show more I conducted growth promotion testing on bacterial and fungal strains including rehydrating organisms and maintaining stock cultures. Managed day to day running of the lab such as preparation and maintenance of cultures and medias, standardization of bacterial (anaerobic and aerobic) and fungal species for different experiments, MIC testing, biomass quantification, bacterial quantification and Alamar blue assay. I have performed molecular biology scientific research using methods such as live/dead qPCR, DNA and RNA extractions, cDNA synthesis. Gained experience with different cell culture lines including passaging, maintaining cell line, media preparation, thawing cryovials and freezing cells. Show less I conducted growth promotion testing on bacterial and fungal strains including rehydrating organisms and maintaining stock cultures. Managed day to day running of the lab such as preparation and maintenance of cultures and medias, standardization of bacterial (anaerobic and aerobic) and fungal species for different experiments, MIC testing, biomass quantification, bacterial quantification and Alamar blue assay. I have performed molecular biology scientific research using methods such as… Show more I conducted growth promotion testing on bacterial and fungal strains including rehydrating organisms and maintaining stock cultures. Managed day to day running of the lab such as preparation and maintenance of cultures and medias, standardization of bacterial (anaerobic and aerobic) and fungal species for different experiments, MIC testing, biomass quantification, bacterial quantification and Alamar blue assay. I have performed molecular biology scientific research using methods such as live/dead qPCR, DNA and RNA extractions, cDNA synthesis. Gained experience with different cell culture lines including passaging, maintaining cell line, media preparation, thawing cryovials and freezing cells. Show less
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Maharashtra University of Health Sciences
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India
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Higher Education
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100 - 200 Employee
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General Dentist
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Sep 2019 - Sep 2020
Conducted thorough examinations, X-Rays, and screenings, identifying the cause of the issue to implement treatment plan. Performed routine restorative procedures minor surgical extractions, prosthodontic procedures, local anesthesia, suture placements/removals in adult and pediatric patients. Educated patients on oral hygiene management for optimal care and disease prevention. Conducted thorough examinations, X-Rays, and screenings, identifying the cause of the issue to implement treatment plan. Performed routine restorative procedures minor surgical extractions, prosthodontic procedures, local anesthesia, suture placements/removals in adult and pediatric patients. Educated patients on oral hygiene management for optimal care and disease prevention.
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Education
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University of Glasgow
Master of Science - MSc, Advanced/Graduate Dentistry and Oral Sciences -
Maharashtra University of Health Sciences
Bachelor of Dental Surgery, Dentistry -
Indian Educational School, Kuwait.
High School Diploma