Experience

ExcellGene SA

• Switzerland
• Biotechnology Research
• 1 - 100 Employee

• Cell Line Development Associate

  • Feb 2022 - Present

  Development of monoclonal stable cell line from CHO cells in order to produce high yield of recombinant proteins: cell culture, transfection, single cell cloning, cell line optimization… Development of monoclonal stable cell line from CHO cells in order to produce high yield of recombinant proteins: cell culture, transfection, single cell cloning, cell line optimization…



Medicover

• Sweden
• Hospitals and Health Care
• 700 & Above Employee

• Laboratory Supervisor

  • Jun 2021 - Feb 2022

  Installation and supervision on-site of a laboratory for Covid-19 diagnostics with three technicians. Management of the workflow (devices and samples), troubleshooting, planning, inventory and implementation of the quality management system (SOPs, CAPAs, EQA, …). Installation and supervision on-site of a laboratory for Covid-19 diagnostics with three technicians. Management of the workflow (devices and samples), troubleshooting, planning, inventory and implementation of the quality management system (SOPs, CAPAs, EQA, …).



Fast Track Diagnostics - A Siemens Healthineers Company

• Research and Development Technician

  • Jan 2019 - May 2021

  Team "Development & Innovation Projects" Research/feasibility, verification and validation (clinical study) phases for the development of real-time multiplex PCR kits for infectious diseases detection. Troubleshooting. Team "Development & Innovation Projects" Research/feasibility, verification and validation (clinical study) phases for the development of real-time multiplex PCR kits for infectious diseases detection. Troubleshooting.



Cell and Gene Therapy Unit, Hospital of Nantes

• Research and Development Technician

  • Nov 2017 - Jun 2018

  Cell culture of cell lines and primary cells of fibroblasts and keratinocytes, cell based assays (in vitro and ex vivo) for a research and development project (RHU Project 5). Unit certified ISO9001 and GMP's Cell culture of cell lines and primary cells of fibroblasts and keratinocytes, cell based assays (in vitro and ex vivo) for a research and development project (RHU Project 5). Unit certified ISO9001 and GMP's



Inserm

• France
• Research Services
• 700 & Above Employee

• Research Associate

  • Jun 2016 - Mar 2017

  Study of drugs toxicity on hepatocytes and their mechanisms of action as part of the European MIP-DILI (Mechanism-Based Integrated Systems for the Prediction of Drug-Induced Liver Injury) project: - Cell culture and cell-based assays (primary cells and HepaRG cell line) - Biochemistry: Western-Blotting, ELISA’s, immunolabelling, enzymatic activity measurement and biochemical assays with spectrophotometer and spectrofluorometer - Molecular biology: RT-qPCR, transfection (basics) - HPLC (basics) See publication below. Show less



Biopredic International

• France
• Biotechnology Research
• 1 - 100 Employee

• Quality Control Technician

  • Apr 2015 - Apr 2016

  Quality control of cell cultures (hepatocytes) and subcellular fractions performing studies of cell seeding, morphology, viability, enzymatic activity measurement and biochemical assays. It also included: - Involvement in R&D projects and activities (see poster below). - Involvement in Production of subcellular fractions. - Replacement of the quality control manager during holidays. - Data management and analysis as well as stock management. Quality control of cell cultures (hepatocytes) and subcellular fractions performing studies of cell seeding, morphology, viability, enzymatic activity measurement and biochemical assays. It also included: - Involvement in R&D projects and activities (see poster below). - Involvement in Production of subcellular fractions. - Replacement of the quality control manager during holidays. - Data management and analysis as well as stock management.



Canada

• United States
• Entertainment Providers
• 1 - 100 Employee

• Working Holidays Visa

  • Apr 2014 - Feb 2015

  Travel and discovery of the country working time to time in different fields. Travel and discovery of the country working time to time in different fields.



Eurofins Group

• Singapore
• Chemical Manufacturing
• 1 - 100 Employee

• Laboratory Technician

  • May 2013 - Mar 2014

  Capacitation to make all kind of microbiological media in aseptic conditions with GLP, 5S and quality requirement respect. Including punctual management of the service (five technicians) when there was no supervisor during the week-end. And work with internal and external audit conditions several times. Further activities: - Daily basis use of autoclaves, hood ans MSCA. - Cleaning and decontamination cycles as well as the calibration of the equipment. - Management of the stocks of products, the engines cycles and the batch liberation through the software « Lims ». Show less



Laboratory of culture and research about algae

• Study Engineer in environmental biotechnologies (internship)

  • Jan 2012 - Jun 2012

  Study of the bacterial diversity associated to three microalgae species and their interactions for an « antifouling application ». - Culture and maintenance of the algal and bacterial strains. - Set up of a DGGE-PCR protocol (also including basic PCR protocol and DNA gel electrophoresis). - Training and capacitation to use a scanning electron microscopy. - Establishment of biotest on microplates. Study of the bacterial diversity associated to three microalgae species and their interactions for an « antifouling application ». - Culture and maintenance of the algal and bacterial strains. - Set up of a DGGE-PCR protocol (also including basic PCR protocol and DNA gel electrophoresis). - Training and capacitation to use a scanning electron microscopy. - Establishment of biotest on microplates.



Eurofins

• Luxembourg
• Biotechnology Research
• 700 & Above Employee

• Laboratory Technician in Chemistry

  • Jul 2011 - Sep 2011

  Analyze of fats and cellulose in food matrix with GLP, 5S and quality requirement respect. Further activities: - Cleaning and decontamination cycles as well as the calibration of the equipment. - Management the stocks of products, the engines cycles and the resulting data via the software « Lims » to make daily report to the supervisor. Analyze of fats and cellulose in food matrix with GLP, 5S and quality requirement respect. Further activities: - Cleaning and decontamination cycles as well as the calibration of the equipment. - Management the stocks of products, the engines cycles and the resulting data via the software « Lims » to make daily report to the supervisor.



Laboratory of life sciences of Heriot-Watt

• Study Engineer in Biochemistry (internship)

  • Mar 2011 - Jun 2011

  Characterization of a protein coming from genetically modified barley. - Set up of a western-blotting protocol. - Set up of an affinity chromatography. - Bacterial transformation. Characterization of a protein coming from genetically modified barley. - Set up of a western-blotting protocol. - Set up of an affinity chromatography. - Bacterial transformation.



CBAC Laboratory (Biological Sensor for Analysis and Control) (UMR-CNRS-GEPEA)

• Study Engineer in Microbiology (internhip leading to job)

  • Mar 2009 - Aug 2009

  Development, set up and realization of microbiological test of biodegradation of xenobiotics. Further activities: - Culture and maintenance of bacterial strains. - Managed the resulting data, analysed the results and reported them to the researcher. - Cleaning and decontamination cycles as well as the calibration of the equipment. Development, set up and realization of microbiological test of biodegradation of xenobiotics. Further activities: - Culture and maintenance of bacterial strains. - Managed the resulting data, analysed the results and reported them to the researcher. - Cleaning and decontamination cycles as well as the calibration of the equipment.