Lucie Charmier
Biochemistry Specialist (R&D) at Megazyme- Claim this Profile
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Portuguese Native or bilingual proficiency
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French Native or bilingual proficiency
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English Full professional proficiency
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Spanish Elementary proficiency
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Bio
João Anes
It’s rare when you come across a standout person like Lucie but it happens. I had the pleasure of working with her for almost one year at University College Dublin, collaborating on several projects. She was an excellent master student, fast learner and the job would get done regardless. Her contagious positive energy is still missed today! I' sure any employee would be lucky to have her in her team.
João Anes
It’s rare when you come across a standout person like Lucie but it happens. I had the pleasure of working with her for almost one year at University College Dublin, collaborating on several projects. She was an excellent master student, fast learner and the job would get done regardless. Her contagious positive energy is still missed today! I' sure any employee would be lucky to have her in her team.
João Anes
It’s rare when you come across a standout person like Lucie but it happens. I had the pleasure of working with her for almost one year at University College Dublin, collaborating on several projects. She was an excellent master student, fast learner and the job would get done regardless. Her contagious positive energy is still missed today! I' sure any employee would be lucky to have her in her team.
João Anes
It’s rare when you come across a standout person like Lucie but it happens. I had the pleasure of working with her for almost one year at University College Dublin, collaborating on several projects. She was an excellent master student, fast learner and the job would get done regardless. Her contagious positive energy is still missed today! I' sure any employee would be lucky to have her in her team.
Experience
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Megazyme
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Ireland
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Biotechnology Research
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1 - 100 Employee
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Biochemistry Specialist (R&D)
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Jan 2016 - Present
The main tasks and responsibilities involved are: -Production and purification of non-GMO enzymes through conventional purification methods (chromatography) - Involved in research projects as directed by the CEO - Proficient in analytical equipment such as HPLC, Dionex, GC and GPC. - Stability studies and biochemical characterisation of enzymes. - Design of novel enzymatic test kits/products and their optimisation - Quality Control of new and existing products. - Preparation of written reports, maintenance and update of SOPs. - Laboratory maintenance and upkeep of all validation/ calibration records (ISO) Show less
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University College Dublin
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Ireland
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Higher Education
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700 & Above Employee
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Internship in Virology - Infection Molecular Biology
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Oct 2015 - Jan 2016
HTLV-1 internship with relevant techniques in:- Cell culture (HEK 293T cells maintenance)- Protein expression in mammalian systems (Transfection and Lysis)- Protein quantification and reporter gene expression (Luciferase assay, BCA assay)- Protein purification and isolation (SDS-PAGE, Western Blot)- Plasmid expression and purification
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MSc Thesis in UCD Centre for Food Safety
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Sep 2014 - Sep 2015
1 year research thesis with relevant experience in:- Project management- Scientific writing and development/optimisation of protocols- Biological Data Analysis- Laboratory maintenance (autoclave waste, labelling and preparing samples, storage maintenance and procurement)- Cell culture (THP-1 cell line)- Cell based bioassays ( Cytotoxicity, cell infection)- Immunoassays (ELISA)- Fluorometric and Spectrophotometric analysis (enzymatic reactions)- Basic Microbiology (Food-borne Pathogens- disk diffusion, MIC, growth curves, swim-swarm)´ Show less
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Internship in Molecular Microbiology
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Mar 2014 - Jul 2014
Internship in Molecular Microbiology: - Basic Microbiology (Disk Diffusion, MIC/MBC) - Molecular Microbiology (Electroporation, plasmid preparation, PFGE, PCR, electroforesis) Internship in Molecular Microbiology: - Basic Microbiology (Disk Diffusion, MIC/MBC) - Molecular Microbiology (Electroporation, plasmid preparation, PFGE, PCR, electroforesis)
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Education
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2013 - 2015
Universidade Nova de Lisboa
Master's degree, Biotechnology -
2010 - 2013
Universidade Lusófona de Humanidades e Tecnologias
Engineer, Biotechnology
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