Experience

HUMANOID: Human Organoid Center of Research Excellence (CoRE)

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Staff

  • Jul 2019 - Jul 2021

  San Diego, California, United States I was an original lab member that helped establish the first organoid biobank at UCSD. I worked to develop a human stem cell-derived complete lung organoid model to research lung disease in COVID-19, performed quality control monitoring of organoid lines across multiple passages and characterization via RNA isolation and RT-PCR, flow cytometry, immunocytochemistry, and confocal microscopy. I prepared dissociated organoids in transwells for SARS-CoV-2 inoculation and and drug screening… Show more I was an original lab member that helped establish the first organoid biobank at UCSD. I worked to develop a human stem cell-derived complete lung organoid model to research lung disease in COVID-19, performed quality control monitoring of organoid lines across multiple passages and characterization via RNA isolation and RT-PCR, flow cytometry, immunocytochemistry, and confocal microscopy. I prepared dissociated organoids in transwells for SARS-CoV-2 inoculation and and drug screening assays. I oversaw virus production and expansion of human Coronavirus OC43 and 229E and infection of lung organoids in air-liquid interface culture. I also isolated human gastrointestinal organoid lines from Crohn’s Disease and Ulcerative Colitis patient biopsies, cultured dissociated organoids in transwells and took transepithelial resistance measurements I created new myofibroblast cell lines, designed a multicellular model of fibrotic Crohn’s disease, and performed assays to optimize growth media. I transdifferentiated SPT6 depleted keratinocytes to a gut-like lineage to recapitulate Barrett's metaplasia Overall my work required that I maintain detailed records of experiments, analyze experimental data, prepare publication quality images of cells and organoids captured via confocal microscopy, and consistently present my progress in weekly lab meetings. I also wrote new protocols and standard operating procedures, and trained new lab members in organoid culture Show less



Cell Applications, Inc.

• United States
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Cell Culture Technician

  • Jul 2018 - Jul 2021

  Greater San Diego Area Our goal at Cell Applications is to provide quality primary cell lines and custom products to researchers and innovators in the biopharmaceutical industry and academia. As a cell culture technician, I am responsible for isolating, culturing and harvesting, and performing quality control assessments of primary cell lines. I have worked extensively with human GI organoids and neural stem cells, as well as over 20 different human, rat and monkey neuronal cell types. I have optimized… Show more Our goal at Cell Applications is to provide quality primary cell lines and custom products to researchers and innovators in the biopharmaceutical industry and academia. As a cell culture technician, I am responsible for isolating, culturing and harvesting, and performing quality control assessments of primary cell lines. I have worked extensively with human GI organoids and neural stem cells, as well as over 20 different human, rat and monkey neuronal cell types. I have optimized microdissection and isolation techniques for 7 different types of embryonic rat neurons. I have also gained experience in RNA isolation and characterization via gel electrophoresis and immunocytochemistry and fluorescent imaging. Another important aspect of my role at Cell Applications is record keeping and data entry. I have developed excellent organizational skills, as I must maintain, collect, and record data on up to 15 different cell lines/lots at a time. Show less



Gateway Genomics, LLC.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Intern

  • Jun 2017 - Jul 2018

  Greater San Diego Area


Scripps Research
• United States
• Research Services
• 700 & Above Employee
• Research Assistant

  • Jun 2017 - Apr 2018