Federico D'Alessio

GMP Downstream Process Development Scientist at Takis Biotech s.r.l.
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Contact Information
us****@****om
(386) 825-5501
Location
Lanuvio, Latium, Italy, IT
Languages
  • Italiano Native or bilingual proficiency
  • Inglese Professional working proficiency

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Giulio Messori

I had the pleasure of working closely in group with Federico at Fondazione Golinelli, during a project where we were asked to plan, as external consultant, a Brand Identity Strategy for an italian phamaceutical company. Federico is a good team member. I enjoyed working with him and I would be more than happy to work with him again in the future.

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Credentials

  • Abilitazione Biologo sez. A
    Ordine Nazionale dei Biologi
    Jul, 2020
    - Sep, 2024
  • Entrepreneurship ICARO 2016
    .Bestr
    Nov, 2016
    - Sep, 2024

Experience

  • Takis Biotech s.r.l.
    • GMP Downstream Process Development Scientist
      • Mar 2021 - Present

      Downstream process development for personalized plasmid vaccines; DNA purification using TFF (tangential flow filtration) and AKTA chromatographic system; HPLC analytical method development for quality control; Optimization of mRNA in-vitro trascription reaction (IVT); mRNA purification using chromatography (FPLC); Execution of release test as indicated in the European Pharmacopoeia. Downstream process development for personalized plasmid vaccines; DNA purification using TFF (tangential flow filtration) and AKTA chromatographic system; HPLC analytical method development for quality control; Optimization of mRNA in-vitro trascription reaction (IVT); mRNA purification using chromatography (FPLC); Execution of release test as indicated in the European Pharmacopoeia.

  • Cantina Il Bottino
    • Business Development
      • Jul 2018 - Present

      Analisi del mercato e sviluppo di nuovi prodotti; Ricerca nuovi clienti; Sviluppo rete commerciale; Analisi del mercato e sviluppo di nuovi prodotti; Ricerca nuovi clienti; Sviluppo rete commerciale;

  • Sapienza University of Rome
    • Italy
    • Higher Education
    • Ph.D. Student
      • Nov 2017 - Dec 2020

      Research activity on vitamin B6 metabolism in E. coli, in particular on the role of YggS in the homeostasis of vitamin B6 and on the possible transfer mechanism of vitamin B6 to PLP-dependent enzymes such as serine hydroxymethyltransferase. Most used techniques: qRT-PCR SDS-PAGE Liquid cromatography UV-Vis spectrophotometry Spectrofluorometry Research activity on vitamin B6 metabolism in E. coli, in particular on the role of YggS in the homeostasis of vitamin B6 and on the possible transfer mechanism of vitamin B6 to PLP-dependent enzymes such as serine hydroxymethyltransferase. Most used techniques: qRT-PCR SDS-PAGE Liquid cromatography UV-Vis spectrophotometry Spectrofluorometry

  • Association "L'acchiappasogni"
    • Tutor of Chemistry
      • Feb 2019 - Jul 2019

      General, Organic and Analytical chemistry teaching activity for students using personalized approaches General, Organic and Analytical chemistry teaching activity for students using personalized approaches

  • Cyprus University of Technology
    • Cyprus
    • Higher Education
    • 400 - 500 Employee
    • Erasmus+ internship
      • Apr 2017 - Jul 2017

      Design of experiments for the production of biogas at alkaline pH and isolation of halo-alkalophilic microorganism: -Biogas analysis using the GC-TCD (Gas Chromatograph); -VFA (Volatile Fatty Acids) analysis using the HPLC. Design of experiments for the production of biogas at alkaline pH and isolation of halo-alkalophilic microorganism: -Biogas analysis using the GC-TCD (Gas Chromatograph); -VFA (Volatile Fatty Acids) analysis using the HPLC.

  • University of Bologna
    • Italy
    • Book and Periodical Publishing
    • 1 - 100 Employee
    • Internship Student
      • Mar 2016 - Mar 2017

      -Extraction and analysis on SDS-PAGE (also 2D) of microbial proteins;-Liquid chromatographic purification (FPLC) and characterization of proteins using UV-Vis spectrophotometer:-Kinetic growth characterization of the bacterium Deinococcus radiodurans.

    • Tutor di Biotecnologie Farmaceutiche
      • Feb 2016 - Oct 2016

      The tutor links the students, the teachers and the administration secretariat.My main roles were:-Support the degree course coordinator;-Diffusion of information about integrative activities to the students;-Mediation of the relationship between the parts (students and teachers);-Information service for incoming students;-Administration of tests about the didactic activity.

  • Theras Group
    • Romania
    • Human Resources Services
    • 1 - 100 Employee
    • External brand consultant
      • Feb 2016 - Oct 2016

      -Analysis of the Brand Identity; -Development of a social media marketing strategy. During the project "ICARO, entrepreneurship school" (Fondazione Golinelli's project) I worked on the main topic on top group-working with other four people who have very different background. -Analysis of the Brand Identity; -Development of a social media marketing strategy. During the project "ICARO, entrepreneurship school" (Fondazione Golinelli's project) I worked on the main topic on top group-working with other four people who have very different background.

  • Sapienza Università di Roma
    • Italy
    • Research Services
    • 700 & Above Employee
    • Internship Student
      • Jun 2014 - Sep 2014

      -Site-specific mutagenesis using PCR; -Gene cloning, microbial transformation in E. coli and gene expression; -Analysis of the protein using SDS-PAGE; -Protein purification with fast protein liquid chromatography method (FPLC). -Site-specific mutagenesis using PCR; -Gene cloning, microbial transformation in E. coli and gene expression; -Analysis of the protein using SDS-PAGE; -Protein purification with fast protein liquid chromatography method (FPLC).

Education

  • Sapienza University of Rome
    Ph.D. in Biochemistry
    2017 - 2020
  • Università di Bologna
    Master's degree, Molecular and Industrial Biotechnology
    2014 - 2016
  • Sapienza Università di Roma
    Bachelor's degree, Agro-Industrial Biotechnology
    2011 - 2014

Community

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