David Xia-Zhu

Clinical Laboratory Scientist - Reporting at Lucence
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Contact Information
Location
US
Languages
  • English Native or bilingual proficiency
  • Mandarin Limited working proficiency
  • Cantonese Limited working proficiency

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Experience

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Clinical Laboratory Scientist - Reporting
      • May 2022 - Present

      • Assessed variant-gene and data review using IGV, ClinVar, gnomAD, COSMIC, Varsome, and in-house database• Curated text for gene-disease and drug association using Clinical Trials, NCCN Guidelines, and FDA Approvals• Evaluated published literature and clinical data for clinical interpretation and automated report generation• Submitted reports for CAP and CLIA proficiency testing

    • Clinical Research Associate
      • Jul 2021 - May 2022

      • Drafted internal and external proposals for clinical trials for genetic profile assays for target oncology populations• Coordinated RNA stability test from 16 patient samples to assess RNA degradation over time and temperature • Set up vendor relationships and contracts for the clinical and research lab, coordinating procurement and finance• Performed high-complexity molecular techniques to detect cfDNA and cfRNA oncogenic mutations

    • Clinical Laboratory Assistant
      • Dec 2020 - Jul 2021

      • Conducted genetic testing for circulating tumor DNA in blood samples including, sample accessioning, plasma extraction, PCR library preparation (AmpliMARK), qPCR, and Illumina sequencing• Curated data for cancer causing mutations in genes and FDA-approved drug therapies

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Laboratory Technician
      • Jul 2020 - Nov 2020

      • Extracted RNA and ran qPCR tests for COVID-19 nasal and oral swabs • Reported COVID-19 test results to patients within a 36-hour turn around time • Conducted general instrument maintenance and lab operations • Extracted RNA and ran qPCR tests for COVID-19 nasal and oral swabs • Reported COVID-19 test results to patients within a 36-hour turn around time • Conducted general instrument maintenance and lab operations

    • United States
    • Biotechnology Research
    • 700 & Above Employee
    • Associate Manufacturing Technician
      • May 2020 - Jul 2020

      • Synthesized oligonucleotides and custom primers for COVID-19 test kits, genome sequencing, and genotyping • Desalted and prepped oligonucleotides for purification and special handling using spectroscopy and organic chemistry • Synthesized oligonucleotides and custom primers for COVID-19 test kits, genome sequencing, and genotyping • Desalted and prepped oligonucleotides for purification and special handling using spectroscopy and organic chemistry

    • Biotechnology Research
    • 1 - 100 Employee
    • Summer Student Researcher
      • Jun 2019 - Aug 2019

      • Performed Western Blot to probe for AF1q and cell cycle related proteins to determine lentivirus liability • Conducted senescence associated beta-galactosidase and crystal violet staining on humane prostate cancer cells • Presented a poster on proliferation of humane prostate cancer cells in relation to AF1q silencing • Performed Western Blot to probe for AF1q and cell cycle related proteins to determine lentivirus liability • Conducted senescence associated beta-galactosidase and crystal violet staining on humane prostate cancer cells • Presented a poster on proliferation of humane prostate cancer cells in relation to AF1q silencing

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Research Assistant
      • Feb 2018 - May 2019

      • Studied Lrtm1, Rpl3, and TR-α genes in mice to correlate thyroid hormone productivity and cardiovascular regeneration • Genotyped and analyzed sequencing data for CRISPR/Cas9 gene knockout mice using PCR, NGS, and digestion • Cultured HEK293 colonies to create plasmid infused adenovirus to prepare for CRISPR/Cas9 gene knockout in mice • Conducted immunostaining and advanced microscopy techniques for single-cell analysis of cardiomyocytes • Studied Lrtm1, Rpl3, and TR-α genes in mice to correlate thyroid hormone productivity and cardiovascular regeneration • Genotyped and analyzed sequencing data for CRISPR/Cas9 gene knockout mice using PCR, NGS, and digestion • Cultured HEK293 colonies to create plasmid infused adenovirus to prepare for CRISPR/Cas9 gene knockout in mice • Conducted immunostaining and advanced microscopy techniques for single-cell analysis of cardiomyocytes

    • Summer Student Researcher
      • Jun 2018 - Mar 2019

      • Used R to model the potential risk factors of venous thromboembolism (VTE) in pediatric oncology patients • Conducted retrospective research on 800 pediatric patients collecting 2000-2015 data from MediTech and EPIC • Presented a poster on the risk factors of VTE, correlating obesity, chemotherapies, and catheter procedures • Used R to model the potential risk factors of venous thromboembolism (VTE) in pediatric oncology patients • Conducted retrospective research on 800 pediatric patients collecting 2000-2015 data from MediTech and EPIC • Presented a poster on the risk factors of VTE, correlating obesity, chemotherapies, and catheter procedures

    • United States
    • Higher Education
    • 700 & Above Employee
    • Lab Intern
      • Sep 2017 - Dec 2017

      • Assisted professors in instructing UC Berkeley’s Introduction to Biology (BIO 1A) course, teaching key lab procedures such as PCR & complementation, enzyme kinetics, biochemical techniques, physiology, and dissection• Contributed evaluations to lab instruction and procedures to improve the course’s exams and coursework

    • Undergraduate Research Assistant
      • Jan 2017 - Aug 2017

      • Analyzed genomic evidence of mammalian loss of pineal glands and obstructions to pineal melatonin synthesis• Utilized bioinformatics and analyses to set up sequencing comparison data in genomic software, Geneious• Researched evolutionary traits of pineal and retinal melatonin pathways and natural selected characteristics of mammalian pineal glands, specializing in marine mammals

Education

  • University of California, Berkeley
    Bachelor’s Degree, Biochemistry and Molecular Biology
    2016 - 2020

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