Alexander Milot

Associate Scientist at Dewpoint Therapeutics
  • Claim this Profile
Contact Information
Location
Boston, Massachusetts, United States, US

Topline Score

Topline score feature will be out soon.

Bio

Generated by
Topline AI

You need to have a working account to view this content.
You need to have a working account to view this content.

Experience

    • United States
    • Biotechnology Research
    • 100 - 200 Employee
    • Associate Scientist
      • Sep 2021 - Present
    • Research Associate II
      • May 2020 - Jun 2021
    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Research Associate
      • Feb 2019 - Feb 2020

      Accountable for target validation and cell culture in many cancer and primary cell lines using very thorough methods and check lists for expression and presence of the target biology and responsiveness to compound treatment. Cells were used in siRNA knockdowns, CRISPR, compound treatment, etc. Many possible oncological indications were tested using compounds for proliferation assays, 3D sphereoid assays and viability assays. Nanostring, q-RTPCR, Incucyte plating, and western blotting were used examine changes in target miRNAs and target pathway genes. Data generated was shared and presented regularly in order to develop further experiments and aid in-vivo work. Show less

    • United States
    • Biotechnology Research
    • 100 - 200 Employee
    • Research Associate
      • Jun 2018 - Jan 2019

      Responsible for target validation and cell culture in a wide variety of cancer cell lines using siRNA knockdowns, peptide treatments, and other experiments. Experiments included testing compounds for activity in reporter assays, proliferation assays and using techniques such as q-RTPCR, western blotting among others to demonstrate levels of desired anti-proliferative of differentiating target effects. Experimental methods and results were then presented in meetings for critique, advice on direction, and keeping others on the project updated. Working closely with the chemistry team was also instrumental to moving forward. Show less

    • United States
    • Biotechnology Research
    • Graduate Student Researcher
      • Jul 2017 - May 2018

      • Researching and developing a Master’s project, experiments and SOPs focused on hepatotoxic idiosyncratic drug’s mechanism of action in liver cell culture lines as wells as primary cell cultures and co-cultures. • Using primary hepatocytes and stellates in culture and co-culture on a new quartz crystal matrix system to generate a novel model for fibrosis and ECM effect on these cultures in 8 day long experiments with frequent monitoring • Involved in optimization, testing, and validation of the QCM system, proving its various capabilities as a physical cellular biosensor in response to different models and experimental conditions. • Generating quantitative data while collaborating cross institutions to present at various conferences in poster and presentation form Show less

    • Hospitals and Health Care
    • 1 - 100 Employee
    • Food and Nutrition Host
      • Sep 2013 - May 2018

      Working directly with the dietitians, appropriate diets and meal s are determined and modified for each daily meal to suit the medical condition of patients. Working with different restrictions, service and options are provided to allow for patients to have options for each meal. Any nutritional supplements are also included and integrated into their meals and diets. It is a fast paced and highly adaptive environment that requires multitasking and attention to detail. Working directly with the dietitians, appropriate diets and meal s are determined and modified for each daily meal to suit the medical condition of patients. Working with different restrictions, service and options are provided to allow for patients to have options for each meal. Any nutritional supplements are also included and integrated into their meals and diets. It is a fast paced and highly adaptive environment that requires multitasking and attention to detail.

Education

  • University of Massachusetts Lowell
    2013 - 2018
  • University of Massachusetts Lowell
    Master's degree, Biotechnology
    2013 - 2018

Community

You need to have a working account to view this content. Click here to join now