Experience

Volastra Therapeutics
• United States
• Biotechnology
• 1 - 100 Employee
• Senior Research Associate

  • Nov 2021 - Present

Stelexis Therapeutics
• United States
• Biotechnology
• 1 - 100 Employee
• Research Scientist

  • Jan 2020 - Oct 2021


ImClone Systems, a wholly-owned subsidiary of Eli Lilly and Company

• Biotechnology Research
• 200 - 300 Employee

• Senior Research Associate

  • Jul 2010 - Jan 2020

  • Member of virus core that provides virus to entire company. Responsible for scale up, purification and titer of virus. Purified virus by cesium chloride as well as Clontech’s AdenoX Mega purification Kits and ABM’s Add-N Pure Kits. Titered virus using the Contech’s AdenoX Rapid titer Kit. Maintained hundreds of plates of cells per week for virus scale up. • Responsible for functional assays of group’s target project, that includes evaluation of several assays as well as optimization… Show more • Member of virus core that provides virus to entire company. Responsible for scale up, purification and titer of virus. Purified virus by cesium chloride as well as Clontech’s AdenoX Mega purification Kits and ABM’s Add-N Pure Kits. Titered virus using the Contech’s AdenoX Rapid titer Kit. Maintained hundreds of plates of cells per week for virus scale up. • Responsible for functional assays of group’s target project, that includes evaluation of several assays as well as optimization. Developed and troubleshot several functional assays including but not limited to migration, spheroid and sprouting assays specific to the needs of the group. • Extensive Mammalian cell culture: transfections, stable clone selection • Molecular biology techniques: DNA/RNA isolations, siRNA • Protein expression, SDS-Page, immunoblot • Maintained detailed, organized and updated lab notebook • Lab Maintenance: inventory, purchasing, reagent prep, etc. • Provide written and oral communication of work • Attend and participate in project team and group meetings • Instrumentation used includes: Invitrogen’s Countess Cell Counter, SpectraMax M4, Nikon Confocal Microscope C1, Omega Luminometer, Zeiss Axiovert 200M, Image Quant 4000, MagMax Preparation System for RNA isolation. Show less • Member of virus core that provides virus to entire company. Responsible for scale up, purification and titer of virus. Purified virus by cesium chloride as well as Clontech’s AdenoX Mega purification Kits and ABM’s Add-N Pure Kits. Titered virus using the Contech’s AdenoX Rapid titer Kit. Maintained hundreds of plates of cells per week for virus scale up. • Responsible for functional assays of group’s target project, that includes evaluation of several assays as well as optimization… Show more • Member of virus core that provides virus to entire company. Responsible for scale up, purification and titer of virus. Purified virus by cesium chloride as well as Clontech’s AdenoX Mega purification Kits and ABM’s Add-N Pure Kits. Titered virus using the Contech’s AdenoX Rapid titer Kit. Maintained hundreds of plates of cells per week for virus scale up. • Responsible for functional assays of group’s target project, that includes evaluation of several assays as well as optimization. Developed and troubleshot several functional assays including but not limited to migration, spheroid and sprouting assays specific to the needs of the group. • Extensive Mammalian cell culture: transfections, stable clone selection • Molecular biology techniques: DNA/RNA isolations, siRNA • Protein expression, SDS-Page, immunoblot • Maintained detailed, organized and updated lab notebook • Lab Maintenance: inventory, purchasing, reagent prep, etc. • Provide written and oral communication of work • Attend and participate in project team and group meetings • Instrumentation used includes: Invitrogen’s Countess Cell Counter, SpectraMax M4, Nikon Confocal Microscope C1, Omega Luminometer, Zeiss Axiovert 200M, Image Quant 4000, MagMax Preparation System for RNA isolation. Show less



Therasis Inc.

• Biotechnology
• 1 - 100 Employee

• Research Associate

  • Mar 2010 - Jul 2010

  • Worked under Head of Cellular Assays, Michela Mattioli, PhD • Worked in the cellular assays facility plating 96 and 384 well sample plates for RNA extraction/ cRNA labeling and microarray analysis using Perkin Elmer FlexDrop Dispense Technology • Evaluated RNA/cRNA concentration and quality using NanoDrop 8000 and Agilent Technology Bioanalyzer respectively • Worked on developing new and modifying high throughput RNA extraction • Extensive Sterile technique, handling several cell… Show more • Worked under Head of Cellular Assays, Michela Mattioli, PhD • Worked in the cellular assays facility plating 96 and 384 well sample plates for RNA extraction/ cRNA labeling and microarray analysis using Perkin Elmer FlexDrop Dispense Technology • Evaluated RNA/cRNA concentration and quality using NanoDrop 8000 and Agilent Technology Bioanalyzer respectively • Worked on developing new and modifying high throughput RNA extraction • Extensive Sterile technique, handling several cell lines at any given time • Represented Cellular Assays group at weekly high throughput planning meetings, and maintained laboratory notebooks and records • Instrumentation used includes Perkin Elmer Fluorescence Plate Reader, Biostack, Beckman Coulter Counter, Perkin Elmer Integration Janus Dispense System, Perkin Elmer Envision 2104 multilabel Reader, KD Biosciences flexiseal • Involved in laboratory operations including ordering supplies, equipment maintenance and upkeep of general lab reagents Show less • Worked under Head of Cellular Assays, Michela Mattioli, PhD • Worked in the cellular assays facility plating 96 and 384 well sample plates for RNA extraction/ cRNA labeling and microarray analysis using Perkin Elmer FlexDrop Dispense Technology • Evaluated RNA/cRNA concentration and quality using NanoDrop 8000 and Agilent Technology Bioanalyzer respectively • Worked on developing new and modifying high throughput RNA extraction • Extensive Sterile technique, handling several cell… Show more • Worked under Head of Cellular Assays, Michela Mattioli, PhD • Worked in the cellular assays facility plating 96 and 384 well sample plates for RNA extraction/ cRNA labeling and microarray analysis using Perkin Elmer FlexDrop Dispense Technology • Evaluated RNA/cRNA concentration and quality using NanoDrop 8000 and Agilent Technology Bioanalyzer respectively • Worked on developing new and modifying high throughput RNA extraction • Extensive Sterile technique, handling several cell lines at any given time • Represented Cellular Assays group at weekly high throughput planning meetings, and maintained laboratory notebooks and records • Instrumentation used includes Perkin Elmer Fluorescence Plate Reader, Biostack, Beckman Coulter Counter, Perkin Elmer Integration Janus Dispense System, Perkin Elmer Envision 2104 multilabel Reader, KD Biosciences flexiseal • Involved in laboratory operations including ordering supplies, equipment maintenance and upkeep of general lab reagents Show less



Pennsylvania State University

• United States
• Higher Education
• 1 - 100 Employee

• Research Technician II/ Laboratory Manager

  • Jun 2009 - Feb 2010

  • Worked under Timothy Ritty, PhD, Associate Professor • Responsible to a Principal Investigator for preparation, analysis and recording of laboratory experiments utilizing SOPs • Responsible for inventorying and ordering laboratory supplies and equipment. Operate laboratory equipment as: spectrophotometer, centrifuge, analytical balance, microscope, liquid scintillation counter, etc. • Prepared various reagents, buffers, and media for experiments as required • Performed surgical… Show more • Worked under Timothy Ritty, PhD, Associate Professor • Responsible to a Principal Investigator for preparation, analysis and recording of laboratory experiments utilizing SOPs • Responsible for inventorying and ordering laboratory supplies and equipment. Operate laboratory equipment as: spectrophotometer, centrifuge, analytical balance, microscope, liquid scintillation counter, etc. • Prepared various reagents, buffers, and media for experiments as required • Performed surgical procedures on experimental mice and/or complex laboratory tests and analysis • Gathered, summarized and recorded data from research projects • Assisted in the instruction of medical and graduate students • Performed literature searches pertaining to new experimental procedures and techniques. Planned experiments and developed several research protocols • Involved in specific projects in the laboratory including investigation of integrin-mediated matrix metalloproteinase (MMP) mechanisms of osteosarcoma metastasis using genetically modified cells and an in-vivo model. Evaluation of cellular attachment to and proliferation on various nano- and micro-scale topologies made with the polymer parylene C. Overexpression of the alpha 3 integrin subunit in osteosarcoma cells Show less • Worked under Timothy Ritty, PhD, Associate Professor • Responsible to a Principal Investigator for preparation, analysis and recording of laboratory experiments utilizing SOPs • Responsible for inventorying and ordering laboratory supplies and equipment. Operate laboratory equipment as: spectrophotometer, centrifuge, analytical balance, microscope, liquid scintillation counter, etc. • Prepared various reagents, buffers, and media for experiments as required • Performed surgical… Show more • Worked under Timothy Ritty, PhD, Associate Professor • Responsible to a Principal Investigator for preparation, analysis and recording of laboratory experiments utilizing SOPs • Responsible for inventorying and ordering laboratory supplies and equipment. Operate laboratory equipment as: spectrophotometer, centrifuge, analytical balance, microscope, liquid scintillation counter, etc. • Prepared various reagents, buffers, and media for experiments as required • Performed surgical procedures on experimental mice and/or complex laboratory tests and analysis • Gathered, summarized and recorded data from research projects • Assisted in the instruction of medical and graduate students • Performed literature searches pertaining to new experimental procedures and techniques. Planned experiments and developed several research protocols • Involved in specific projects in the laboratory including investigation of integrin-mediated matrix metalloproteinase (MMP) mechanisms of osteosarcoma metastasis using genetically modified cells and an in-vivo model. Evaluation of cellular attachment to and proliferation on various nano- and micro-scale topologies made with the polymer parylene C. Overexpression of the alpha 3 integrin subunit in osteosarcoma cells Show less